Epitope mapping of monoclonal antibodies raised to recombinant Mengo 3D polymerase.
Epitope mapping of monoclonal antibodies raised to recombinant Mengo 3D polymerase.
复制标题
使用重组 Mengo 3D 聚合酶产生的单克隆抗体的表位作图。
DOI：
10.1007/bf00568908
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发表时间：
1996
期刊：
影响因子：
1.6
通讯作者：
Palmenberg,AC
中科院分区：
文献类型：
--
作者：
Duque,H;Palmenberg,AC
The cDNA coding sequence of the RNA-dependent RNA polymerase (3Dpol) of Mengovirus was cloned and expressed in a bacterial system. Eleven monoclonal antibodies were raised against the recombinant Mengo 3Dpol(rM3D). All of them recognized the recombinant and the viral-induced form of the protein. The panel of monoclonals belonged to the IgG1 and IgG2a isotypes and were mapped to four different epitopes in the 3D molecule by competition assays. All monoclonals recognized Mengo 3Dpolin western blots and cross-reacted with the homologous polymerases of seven other cardioviruses but failed to react with 3Dpolfrom poliovirus type 1 and 3 or rhinovirus type 14 and 16.
