Specialized patterns of vascular differentiation antigens in the pregnant mouse uterus and the placenta
Specialized patterns of vascular differentiation antigens in the pregnant mouse uterus and the placenta
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DOI：
10.1095/biolreprod61.6.1393
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发表时间：
1999-12-01
影响因子：
3.6
通讯作者：
Butcher, EC
中科院分区：
文献类型：
--
作者：
Kruse, A;Hallmann, R;Butcher, EC
妊娠的成功取决于滋养层细胞渗透母体蜕膜和破坏子宫血管的能力。为了询问血管区和中央底蜕膜中的母体内皮细胞（EC）的抗原表型是否可以反映这些血管与滋养层相互作用的专门编程，我们对几种驼鹿EC分化抗原进行了调查，包括MECA-32，MECA-99和endoglin，我们的研究结果显示，在妊娠第9天和妊娠中期，妊娠子宫的不同隔室中血管内皮衬里的表型存在显著差异。MECA-99和MECA-32在基底蜕膜中央血管和血管带中表达较强，而在包膜中表达较弱。此外，血管区用抗内皮糖蛋白染色明亮。重要的是，浸润的滋养层细胞以及衬于母体血液空间的滋养层细胞是MECA-99（+）、MECA-32（-）和内皮糖蛋白（-），这表明MECA-99的表达可能反映了这些滋养层细胞和EC为未来相互作用的专门的共编程，而且滋养层细胞可能模拟与它们在衬于母体血液空间中的作用相关的内皮的选定抗原特征。在长期妊娠子宫的所有分化抗原的表达显着下降，这表明滋养层细胞以及母体EC失去其选定的抗原特性时，胎盘形成的过程是完整的。
The success of pregnancy depends on the ability of trophoblast cells to infiltrate the maternal decidua and breach uterine vessels. To ask whether the antigenic phenotype of maternal endothelial cells (EC) in the vascular zone and central decidua basalis may reflect a specialized programming of these vessels for interaction with the trophoblast, we did a survey of several moose EC differentiation antigens, including MECA-32, MECA-99, and endoglin, Our results revealed striking differences in the phenotype of endothelial lining of vessels in the distinct compartments of the pregnant uterus during Day 9 of pregnancy and at midgestation. Vessels in the central decidua basalis and the vascular zone showed strong expression of MECA-99 but only weak expression of MECA-32, contrasting with the MECA-99(lo), MECA-32(hi) vessels in the capsularis. The vascular zone in addition stained brightly with anti-endoglin. Importantly, invading trophoblast as well as trophoblast cells lining maternal blood spaces were MECA-99(+), MECA-32(-) and endoglin(-), suggesting that the expression of MECA-99 may reflect a specialized coprogramming of these trophoblast and EC for future interaction, but also that trophoblast cells may mimic selected antigenic characteristics of endothelium in association with their role in lining maternal blood spaces. In the term pregnant uterus the expression of all differentiation antigens decreased dramatically, suggesting that trophoblast cells as well as maternal EC lose their selected antigenic characteristics when the process of placentation is complete.