ANTIVIRAL ACTIVITY OF GLYCYRRHIZIN AGAINST VARICELLA-ZOSTER VIRUS INVITRO
ANTIVIRAL ACTIVITY OF GLYCYRRHIZIN AGAINST VARICELLA-ZOSTER VIRUS INVITRO
复制标题
DOI：
10.1016/0166-3542(87)90025-8
复制
发表时间：
1987-02-01
影响因子：
7.6
通讯作者：
SHIGETA, S
中科院分区：
文献类型：
--
作者：
BABA, M;SHIGETA, S
研究了植物提取物甘草酸(GL)对水痘带状疱疹病毒(VZV)的体外抗病毒作用。人胚胎成纤维细胞接种病毒后(后处理)，GL对5株VZV的平均半数抑制剂量(ID50)为0.71 mm，选择性指数(宿主细胞DNA合成的ID50与VZV复制的ID50之比)为30。GL对HEF细胞接种前24小时的VZV复制也有明显的抑制作用。此外，浓度为2.4 mM的GL在37度条件下，30min内对病毒颗粒的灭活率可达99%以上。与其他抗疱疹药物(阿昔洛韦、阿糖腺苷、溴乙烯基脱氧尿苷和磷酸甲酸盐)或人天然β-干扰素联合使用时，GL对VZV的复制具有相加或轻微的协同作用。其抗VZV的作用机制尚不清楚。我们推测GL可抑制病毒颗粒的穿透、脱膜或释放。
One of the plant extracts, glycyrrhizin (GL) was investigated for its antiviral action on varicella-zoster virus (VZV) in vitro. When human embryonic fibroblast (HEF) cells were treated with GL after inoculation of virus (post-treatment), the average 50%-inhibitory dose (ID50) for five VZV strains was 0.71 mM, and the selectivity index (ratio of ID50 for host-cell DNA synthesis to ID50 for VZV replication) was 30. GL was also effective against VZV replication when HEF cells were treated 24 h before the inoculation (pretreatment). Furthermore, at a concentration of 2.4 mM GL inactivated more than 99% of virus particles within 30 min at 37.degree. C. In combination with other anti-herpes drugs (acyclovir, adenine arabinoside, bromovinyldeoxyuridine, and phosphonoformate) or human native beta-interferon, GL had an additive or slightly synergistic effect on VZV replication. The mechanism of anti-VZV action is still unclear. We postulate that GL inhibits the penetration, uncoating or release of virus particles.