Plunc, a member of the secretory gland protein family, is up-regulated in nasal respiratory epithelium after olfactory bulbectomy
Plunc, a member of the secretory gland protein family, is up-regulated in nasal respiratory epithelium after olfactory bulbectomy
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DOI：
10.1074/jbc.m106208200
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发表时间：
2002-04-12
影响因子：
4.8
通讯作者：
Ronnett, GV
中科院分区：
文献类型：
--
作者：
Sung, YK;Moon, C;Ronnett, GV
减法抑制杂交与高通量筛选用于鉴定嗅球病变后鼻上皮中差异表达的基因转录本，称为嗅球切除术。我们用这种方法分离了一种在肺和鼻咽区高度表达的小鼠基因plunc的大鼠同源物。大鼠plunc编码一种含有270个氨基酸的蛋白质，其中含有一种推测的信号肽。Northern blot和原位杂交证实了呼吸上皮蛋白表达上调。plunc mRNA在成年鼠鼻上皮、心脏、肺、胸腺和唾液腺中均有表达。在胚胎发生过程中，Plunc在鼻上皮、胸腺和唾液腺中表达。抗Plunc抗体在肺、心脏和脾脏中检测到31 kda蛋白。大鼠鼻上皮表现出强烈的免疫反应性，这种反应性高度局限于呼吸上皮的微绒毛层。细胞球切除后，呼吸上皮中plunc的表达上调。我们还在大鼠和人的黏液中检测到了分泌的水柱。序列和同源性分析表明，Plume是分泌腺蛋白家族的成员，可能具有杀菌/抑菌功能。这是在呼吸上皮中发现的第一个表达受嗅觉神经元损伤调节的蛋白，并可能提供保护，防止损伤后的感染。
Subtraction suppression hybridization was used with high throughput screening to identify transcripts of genes that are differentially expressed in nasal epithelium following lesioning of the olfactory bulb, termed bulbectomy. We isolated the rat homologue of plunc, a murine gene highly expressed in lung and nasopharyngeal regions, by this method. Rat plunc encodes a 270-amino acid protein containing a putative signal peptide. plunc up-regulation in respiratory epithelium was confirmed by Northern blot and in situ hybridization. plunc mRNA was expressed in nasal epithelium, heart, lung, thymus, and salivary gland in adult rodent. plunc was expressed in nasal epithelium, thymus, and salivary gland during embryogenesis. Antibodies against Plunc detected a 31-kDa protein in lung, heart, and spleen. Rat nasal epithelium displayed robust immunoreactivity that was highly localized to the microvilli layer of respiratory epithelium. The expression of plunc was upregulated after bulbectomy in respiratory epithelium. We also detected secreted plunc in rat and human mucus. Sequence and homology analyses suggest that Plume is a member of the secretory gland protein family with putative bactericidal/bacteriostatic function. This is the first protein found in respiratory epithelium whose expression is regulated by olfactory neuronal injury and may provide protection against infection subsequent to injury.