Coffee polyphenol caffeic acid but not chlorogenic acid increases 5'AMP-activated protein kinase and insulin-independent glucose transport in rat skeletal muscle.
Coffee polyphenol caffeic acid but not chlorogenic acid increases 5'AMP-activated protein kinase and insulin-independent glucose transport in rat skeletal muscle.
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DOI：
10.1016/j.jnutbio.2011.09.001
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发表时间：
2012-11
期刊：
影响因子：
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通讯作者：
S. Tsuda;T. Egawa;Xiao Ma;Rieko Oshima;Eriko Kurogi;Tatsuya Hayashi
中科院分区：
文献类型：
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作者：
S. Tsuda;T. Egawa;Xiao Ma;Rieko Oshima;Eriko Kurogi;Tatsuya Hayashi
绿原酸是咖啡酸和奎尼酸的酯，并且是最广泛消费的多酚之一，因为它在食物中丰富，特别是咖啡。我们探讨了绿原酸及其代谢产物咖啡酸是否直接作用于骨骼肌，以刺激5′-腺苷一磷酸激活的蛋白激酶（AMPK）。用含咖啡酸（≥0.1 mM，≥30 min）但不含绿原酸的Krebs缓冲液孵育大鼠上滑车肌，以剂量和时间依赖性方式增加AMPKα Thr 172（激酶激活的关键步骤）和乙酰CoA羧化酶Ser 79（AMPK的下游靶点）的磷酸化。对AMPK亚型特异性活性的分析显示AMPKα2活性显著增加，而AMPKα1活性没有变化。这种酶的激活与磷酸肌酸含量的减少和3-O-甲基-D-葡萄糖转运活性在胰岛素的情况下增加的速率。这些结果表明，咖啡酸，而不是绿原酸急性刺激骨骼肌AMPK活性和胰岛素非依赖性葡萄糖转运细胞内能量状态的减少。
Chlorogenic acid is an ester of caffeic and quinic acids, and is one of the most widely consumed polyphenols because it is abundant in foods, especially coffee. We explored whether chlorogenic acid and its metabolite, caffeic acid, act directly on skeletal muscle to stimulate 5′-adenosine monophosphate-activated protein kinase (AMPK). Incubation of rat epitrochlearis muscles with Krebs buffer containing caffeic acid (≥0.1 mM, ≥30 min) but not chlorogenic acid increased the phosphorylation of AMPKα Thr172, an essential step for kinase activation, and acetyl CoA carboxylase Ser79, a downstream target of AMPK, in a dose- and time-dependent manner. Analysis of isoform-specific AMPK activity revealed that AMPKα2 activity increased significantly, whereas AMPKα1 activity did not change. This enzyme activation was associated with a reduction in phosphocreatine content and an increased rate of 3-O-methyl-d-glucose transport activity in the absence of insulin. These results suggest that caffeic acid but not chlorogenic acid acutely stimulates skeletal muscle AMPK activity and insulin-independent glucose transport with a reduction of the intracellular energy status.