HSP 10 is a new autoantigen in both autoimmune pancreatitis and fulminant type 1 diabetes.
HSP 10 is a new autoantigen in both autoimmune pancreatitis and fulminant type 1 diabetes.
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DOI：
10.1016/j.bbrc.2009.06.009
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发表时间：
2009-08
影响因子：
3.1
通讯作者：
Soichi Takizawa;T. Endo;Wanjia Xing;Shoichiro Tanaka;Masashi Takahashi;Tetsuro Kobayashi
中科院分区：
文献类型：
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作者：
Soichi Takizawa;T. Endo;Wanjia Xing;Shoichiro Tanaka;Masashi Takahashi;Tetsuro Kobayashi
为了寻找自身免疫性胰腺炎（AIP）的自身抗原，我们用患者的血清筛选了人胰腺cDNA文库，获得了10个阳性克隆。10个克隆中有7个是淀粉酶α-2A，这是AIP和暴发性1型糖尿病（FT1DM）患者血清中特异性检测到的自身抗体[T]。远藤，田中，高桥，藤井，上泽，小林，淀粉酶α-2A自身抗体在自身免疫性胰腺炎和暴发性1型糖尿病中的应用[j]。对剩余3个阳性克隆中的1个进行测序，发现其与热休克蛋白10 (hsp10) cDNA相同。利用重组热休克蛋白10，我们建立了检测热休克蛋白10自身抗体的酶联免疫吸附试验（ELISA）系统。我们发现抗hsp10的自身抗体在AIP患者（92%）和FT1DM患者（81%）的血清中也有很高的频率，但在慢性酒精性胰腺炎（8%）或健康志愿者（1.4%）的血清中没有。这些结果表明，抗hsp10的自身抗体也是AIP和FT1DM的新诊断标志物。
To search autoantigens in autoimmune pancreatitis (AIP), we have screened the human pancreas cDNA library with a patient’s serum and obtained 10 positive clones. Seven out of 10 clones were amylase α-2A, the autoantibody to which was specifically detected in sera from patients with AIP and fulminant type 1 diabetes (FT1DM) [T. Endo, S. Takizawa, S. Tanaka, M. Takahashi, H. Fujii, T. Kamisawa, T. Kobayashi, Amylase α-2A autoantibodies: novel marker of autoimmune pancreatitis and fulminant type 1 diabetes mellitus, Diabetes 58 (2009) 732–737]. Sequencing of 1 out of remaining 3 positive clones revealed that it was identical to heat shock protein 10 (HSP 10) cDNA. Using a recombinant HSP 10, we have developed enzyme-linked immunosorbent assay (ELISA) system for detecting autoantibodies against HSP 10. We found that autoantibody against HSP 10 was also produced with high frequency in sera from patients with AIP (92%) and FT1DM (81%), but not in chronic alcoholic pancreatitis (8%) or healthy volunteers (1.4%). These results suggest that an autoantibody against HSP 10 is also a new diagnostic marker for both AIP and FT1DM.