Epidermal growth factor augments adaptation following small bowel resection: optimal dosage, route, and timing of administration.
Epidermal growth factor augments adaptation following small bowel resection: optimal dosage, route, and timing of administration.
复制标题
表皮生长因子增强小肠切除术后的适应：最佳剂量、给药途径和时间。
DOI：
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发表时间：
1998
影响因子：
2.2
通讯作者：
B. Warner
中科院分区：
文献类型：
--
作者：
C. Shin;M. Helmrath;R. Falcone;J. Fox;Katherine R. Duane;C. Erwin;B. Warner
背景
在各种小肠切除(SBR)动物模型中，外源性表皮生长因子(EGF)已被证明可增强肠道适应。本研究旨在阐明外源性EGF促进SBR小鼠模型适应的最佳剂量、途径和时机。
方法
雄性ICR小鼠接受50%近端SBR或假手术(肠切除再吻合)，然后随机接受生理盐水或人重组EGF(5，50,150或300微克/公斤/天)，每日两次腹腔注射。注射或经口灌胃(o.g.)7日龄时测定回肠蛋白质和DNA含量、隐窝深度和绒毛高度。然后在适应期(SBR后1个月)或适应期(SBR后1个月)期间(SBR后1周)给药1周。用单因素方差分析方法分析组均数之间的差异。P<；0.05被认为意义重大。
结果
EGF在50微g/kg/d剂量下对DNA和蛋白质含量、隐窝深度和绒毛高度的促进作用最大。路线。当EGF在适应性反应已经发生后给予时，在增强适应性方面没有显著效果。
结论
特定剂量和途径的EGF可最佳地增强肠道适应性。外源性EGF仅在对SBR的适应性反应期间增强适应，而不是在SBR已经发生之后。确定EGF给药的最佳条件将允许系统地了解EGF在肠道适应过程中的有益作用的机制。
BACKGROUND
In assorted animal models of small bowel resection (SBR), exogenous epidermal growth factor (EGF) has been shown to augment intestinal adaptation. This study was designed to elucidate the optimal dose, route, and timing of exogenous EGF to boost adaptation in our murine model of SBR.
METHODS
Male ICR mice underwent either 50% proximal SBR or sham surgery (bowel transection with reanastomosis) and then randomized to receive either saline or human recombinant EGF (5, 50, 150, or 300 microg/kg/day) by twice daily intraperitoneal (i.p.) injection or orogastric gavage (o.g.). At 7 days, protein and DNA content, crypt depth, and villus height were determined in the ileum. The premium dose and route was then given for 1 week either during (1 week after SBR) or after the adaptive phase (1 month after SBR). Differences between group means were analyzed using ANOVA. A P < 0.05 was considered significant.
RESULTS
EGF enhanced DNA and protein content, crypt depth, and villus height to the greatest extent at a dosage of 50 microg/kg/day by the o.g. route. EGF had no significant effect on enhancing adaptation when given after the adaptive response had already occurred.
CONCLUSIONS
Intestinal adaptation is optimally enhanced by a specific dose and route of EGF. Exogenous EGF enhances adaptation only during the adaptive response to SBR and not after it has already taken place. Determination of the best circumstances for EGF administration will permit a systematic approach toward understanding a mechanism for the beneficial effect of EGF during intestinal adaptation.
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影响因子：
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作者：
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DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Scheving,LA;Shiurba,RA;Nguyen,TD;Gray,GM
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