Biological laser printing of three dimensional cellular structures
Biological laser printing of three dimensional cellular structures
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DOI：
10.1007/s00339-004-2620-3
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发表时间：
2004-09-01
影响因子：
2.7
通讯作者：
Ringeisen, BR
中科院分区：
文献类型：
--
作者：
Barron, JA;Spargo, BJ;Ringeisen, BR
本程序介绍了实验证明，BioLP（TM），生物激光打印，可用于快速，准确地打印单个细胞以及三维细胞图案。人类骨肉瘤细胞被沉积到生物聚合物基质中，孵育24小时后，打印的细胞保持活力并繁殖。将多层人骨肉瘤细胞转移到MatrigelTM基质中，产生三维细胞构建体。进行活/死测定，证明在被打印到生物聚合物中之后，大于95%的细胞是活的。选择性打印单个细胞的能力对于细胞选择研究以及基因组学和蛋白质组学研究具有潜在的益处。此外，这种技术可以使组织支架层以极高的精度和分辨率接种细胞。
This proceeding presents experiments demonstrating that BioLP(TM), Biological Laser Printing, can be used to rapidly and accurately print individual cells as well as patterns of cells in three dimensions. Human osteosarcoma cells were deposited into a biopolymer matrix, and after twenty-four hours of incubation, the printed cells remained viable and had multiplied. Multiple layers of human osteosarcoma cells were transferred into a Matrigel(TM) substrate, producing a three-dimensional cellular construct. Live/dead assays were performed, demonstrating that greater than 95% of the cells were viable after the being printed into the biopolymer. The ability to selectively print individual cells has potential benefits for cell selection studies and genomics and proteomics research. In addition, this technique could enable layers of tissue scaffolding to be seeded with cells at exceedingly high precision and resolution.