Development of a Coflowing Device for the Size-Controlled Preparation of Magnetic-Polymeric Microspheres as Embolization Agents in Magnetic Resonance Navigation Technology.
Development of a Coflowing Device for the Size-Controlled Preparation of Magnetic-Polymeric Microspheres as Embolization Agents in Magnetic Resonance Navigation Technology.
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DOI：
10.1021/acsbiomaterials.7b00839
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发表时间：
2018-02
影响因子：
5.8
通讯作者：
Zeynab Nosrati;Ning Li;F. Michaud;Sahan A. Ranamukhaarachchi;Stoyan Karagiozov;G. Soulez;S. Martel;K. Saatchi;U. Häfeli
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文献类型：
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作者：
Zeynab Nosrati;Ning Li;F. Michaud;Sahan A. Ranamukhaarachchi;Stoyan Karagiozov;G. Soulez;S. Martel;K. Saatchi;U. Häfeli
最近已经引入液滴微流体技术来产生用于许多生物医学应用的颗粒，包括肝动脉、子宫动脉或支气管动脉中的治疗性栓塞剂。栓塞剂有多种形状和尺寸，可根据靶血管特征进行调整。磁性栓塞剂可以另外被导航到目标位置（例如，肿瘤）通过血液系统。这项技术被称为磁共振导航（MRN）。在这里，我们介绍了一种高通量的方法来生产均匀尺寸的磁性微球（MMS）作为血管栓塞剂与MRN结合使用。MMS生产系统由一个简单的3D打印微共流装置组成，该装置能够生产生物相容的、降解速率可控的包裹磁性纳米颗粒的聚乳酸-羟基乙酸共聚物（PLGA）微球。轴对称流动是通过中心针注射由连续相包围的分散相来获得的，并且导致形成尺寸受控的液滴，所述液滴变成线性地取决于内针直径的均匀尺寸的MMS。MMS形态、平均粒度和粒度分布由SEM图像定量。利用振动样品磁强计研究了MMS的磁性能。MMS对HUVEC（人脐静脉内皮细胞）和HEK 293（人胚肾）细胞无毒性。本文提出的微共流法可以可靠地制备尺寸为130-700 μm、粒度分布窄（CV < 7%）、磁性能良好的磁性纳米材料。
Droplet microfluidics technology has recently been introduced to generate particles for many biomedical applications that include therapeutic embolizing agents in hepatic, uterine or bronchial arteries. Embolic agents are available in a variety of shapes and sizes that are adjusted according to the target vessel characteristics. Magnetic embolic agents can additionally be navigated to the target location (e.g., a tumor) through the blood system by applying an external magnetic field. This technology is termed Magnetic Resonance Navigation (MRN). Here we introduce a high throughput method to produce homogeneously sized magnetic microspheres (MMS) as blood vessel embolic agents for use in combination with MRN. The system for MMS production consists of a simple 3D printed micro coflowing device that is able to produce biocompatible, degradation rate controllable poly(lactic-co-glycolic acid) (PLGA) microspheres encasing magnetic nanoparticles. Axisymmetric flow is obtained with a central needle injecting the dispersed phase surrounded by a continuous phase and leads to the formation of size-controlled droplets that turn into homogeneously sized MMS linearly dependent on the inner needle diameter. MMS morphology, mean particle size and size distribution were quantified from SEM images. Magnetic performance of MMS was investigated using a vibrating sample magnetometer. MMS were nontoxic toward HUVEC (human umbilical vein endothelial cells) and HEK293 (human embryonic kidney) cells. The presented micro coflowing method allows for the reliable production of large MMS sized 130-700 μm with narrow size distribution (CV < 7%) and magnetic properties useful for MRN.