THE ACTIONS OF INSULIN-LIKE GROWTH FACTOR-I AND FACTOR-II ON CULTURED SERTOLI CELLS
THE ACTIONS OF INSULIN-LIKE GROWTH FACTOR-I AND FACTOR-II ON CULTURED SERTOLI CELLS
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DOI：
10.1210/endo-114-1-240
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发表时间：
1984-01-01
期刊：
影响因子：
4.8
通讯作者：
CZECH, MP
中科院分区：
文献类型：
--
作者：
BORLAND, K;MITA, M;CZECH, MP
从幼龄大鼠（13日龄）培养的支持细胞显示，在微摩尔浓度的胰岛素和纳摩尔浓度的胰岛素样生长因子II （IGF-II）的作用下，[3H]胸腺嘧啶与DNA的结合增加，乳酸的产生增加，[3H]亮氨酸与蛋白质的结合增加。第一种反应也见于纳摩尔浓度的igf - 1。利用Sertoli细胞膜和整个Sertoli细胞进行的受体亲和力标记研究表明，这些细胞具有丰富的I型生长因子受体（MW, 350,000），对IGF-I具有高亲和力，对IGF-II具有中等亲和力，对胰岛素具有低亲和力。支持细胞膜也显示出丰富的II型生长因子受体（MW, 230,000），对IGF-II具有高亲和力，对IGF-I具有中等亲和力，对胰岛素没有可检测到的亲和力。此外，在100 nM或更高的浓度下，观察了Sertoli细胞对胰岛素的反应；已知胰岛素受体在浓度为10纳米或更低时被胰岛素饱和。支持细胞具有igf - 1受体，对胰岛素的反应可能是这些激素与igf - 1受体结合的结果。
Cultured Sertoli cells prepared from young rats (13 days old) showed increased incorporation of [3H]thymidine into DNA, increased production of lactate and increased incorporation of [3H]leucine into protein in response to micromolar concentrations of insulin and nanomolar concentrations of insulin-like growth factor II (IGF-II). The first of these responses was also seen with nanomolar concentrations of IGF-I. Receptor affinity labeling studies using Sertoli cell membranes and whole Sertoli cells showed that these cells possess abundant growth factor receptors of type I (MW, 350,000) that show high affinity for IGF-I, moderate affinity for IGF-II and low affinity for insulin. Sertoli cell membranes also show abundant growth factor receptors of type II (MW, 230,000) that show high affinity for IGF-II, moderate affinity for IGF-I and no detectable affinity for insulin. Moreover, the responses of the Sertoli cell to insulin were observed at concentrations of 100 nM or higher; insulin receptors are known to be saturated by insulin at concentrations of 10 nM or less. Sertoli cells possess receptors for IGF-I and the responses observed to insulin may result from binding of these hormones to receptors for IGF-I.