Cloning and characterization of the human and Caenorhabditis elegans homologs of the Saccharomyces cerevisiae MSH5 gene.
Cloning and characterization of the human and Caenorhabditis elegans homologs of the Saccharomyces cerevisiae MSH5 gene.
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DOI：
10.1006/geno.1998.5447
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发表时间：
1998-10
期刊：
影响因子：
4.4
通讯作者：
N. Winand;Jessica A. Panzer;Richard D. Kolodner
中科院分区：
文献类型：
--
作者：
N. Winand;Jessica A. Panzer;Richard D. Kolodner
在酿酒酵母中，编码MutS同源物的MSH 5基因被鉴定为减数分裂交换所需的基因。为了了解MSH 5在高等真核生物中的作用，我们鉴定了人类和秀丽隐杆线虫的MSH 5基因。人类和C. elegans MSH 5氨基酸序列与S.酿酒酵母MSH 5氨基酸序列。人MSH 5基因由25个外显子组成，基因组DNA至少有12 kb，而C.秀丽隐杆线虫基因由17个外显子组成，分布在至少5.8 kb的区域内。辐射杂交定位研究表明，人类基因位于6p22.3-p21.3。北方印迹分析表明，人MSH 5在所有组织中都有一定程度的表达，但在睾丸、胸腺和免疫系统的其他组织中表达水平特别高。双杂交相互作用分析表明，人MSH 4和MSH 5蛋白相互作用与S.酿酒酵母MSH 4和MSH 5。
In Saccharomyces cerevisiae the MSH5 gene encoding a MutS homolog was identified as a gene required for meiotic crossing over. To understand the role of MSH5 in higher eukaryotes, we have identified both the human and the Caenorhabditis elegans MSH5 genes. The human and C. elegans MSH5 predicted amino acid sequences share, respectively, 25.3 and 22.0% identity with the S. cerevisiae MSH5 amino acid sequence. The human MSH5 gene consists of 25 exons and spans at least 12 kb of genomic DNA, while the C. elegans gene comprises 17 exons distributed over at least 5.8 kb. Radiation hybrid mapping studies indicate that the human gene is located at 6p22.3-p21.3. Northern blot analysis demonstrates that human MSH5 is expressed to some extent in all tissues, but that particularly high levels of expression occur in testis, thymus, and other tissues of the immune system. Two-hybrid interaction analysis demonstrates that the human MSH4 and MSH5 proteins interact as observed for S. cerevisiae MSH4 and MSH5.