Effectiveness of bacteriophage JN01 incorporated in gelatin film with protocatechuic acid on biocontrol of Escherichia coli O157:H7 in beef
Effectiveness of bacteriophage JN01 incorporated in gelatin film with protocatechuic acid on biocontrol of Escherichia coli O157:H7 in beef
复制标题
噬菌体 JN01 与原儿茶酸掺入明胶膜对牛肉中大肠杆菌 O157:H7 的生物防治效果
DOI：
10.1111/ijfs.15673
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发表时间：
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影响因子：
3.3
通讯作者：
Wu Guo-Ping
中科院分区：
文献类型：
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作者：
Li Ya-xing;Zhong Chan;Zhang Hui-Zheng;Zhao Yuan-Yang;Shu Mei;Wu Guo-Ping
用大肠杆菌157:H7噬菌体JN01制备了一种新的明胶-原儿茶酸（PCA）膜并进行了表征。加入JN01后，明胶- pca膜的溶胀值、水蒸气渗透性、水溶性和断裂伸长率无显著差异。JN01的加入提高了膜的值和透明度，降低了膜的值、值和抗拉强度。明胶- pca - jn01膜的抗氧化活性为60.07%。在水溶液中，明胶-JN01和明胶- pca -JN01膜均能稳定释放JN01, 11 h后，其释放率分别为7.56%和0.12%。微观结构分析表明，JN01颗粒在薄膜中呈簇状均匀分布，PCA的存在会减弱这种聚集。与此同时,E。与纯明胶薄膜相比，明胶- jn01和明胶- pca - jn01膜的coliO157:H7计数分别减少1.13 log10CFU mL - 1和0.45 log10CFU mL - 1。在4°C保存7天后，与未包装明胶膜的牛肉相比，包装这两种明胶膜的牛肉中大肠杆菌大肠杆菌157:H7的计数分别减少1.00 log10CFU g−1和0.80 log10CFU g−1。综上所述，所制备的明胶- pca - jn01薄膜在食品保鲜方面具有潜在的应用前景。
A new gelatin-protocatechuic acid (PCA) film withEscherichia coliO157:H7 phage JN01 was developed and characterised. After incorporated with JN01, swelling value, water vapour permeability, water solubility and elongation at break of gelatin-PCA film were not significantly different. The addition of JN01 increasedbvalue and transparency of film, while it decreasedLvalue,avalue and tensile strength of film. Moreover, the gelatin-PCA-JN01 film presented antioxidant activity of 60.07%. Furthermore, JN01 could be steadily released from gelatin-JN01 and gelatin-PCA-JN01 films in aqueous solution, and their release rates were 7.56% and 0.12% after 11 h, respectively. The microstructure analysis showed that JN01 particles were clustered and uniformly distributed in film, and the aggregation would be attenuated in the presence of PCA. Meanwhile,E.coliO157:H7 counts were 1.13 log10CFU mL−1and 0.45 log10CFU mL−1lower in gelatin-JN01 and gelatin-PCA-JN01 films compared with pure gelatin filmin vitroat 4 °C for 24 h, respectively. After stored at 4 °C for 7 days,Escherichia coliO157:H7 counts were 1.00 log10CFU g−1and 0.80 log10CFU g−1lower in beef packed with these two gelatin films, compared with beef without gelatin film, respectively. In conclusion, the developed gelatin-PCA-JN01 film has potential application in food preservation.