Chimerism of Murine Fetal Bone Marrow by Maternal Cells Occurs in Late Gestation and Persists into Adulthood
Chimerism of Murine Fetal Bone Marrow by Maternal Cells Occurs in Late Gestation and Persists into Adulthood
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母体细胞对小鼠胎儿骨髓的嵌合现象发生在妊娠晚期并持续到成年期
DOI：
10.1097/01.lab.0000067500.85003.32
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发表时间：
2003
影响因子：
5
通讯作者：
B. Croy
中科院分区：
文献类型：
--
作者：
A. Marleau;A. Marleau;Janice D. Greenwood;Qingxia Wei;Bhagirath Singh;B. Croy
对在转基因标记的免疫活性母鼠中妊娠的小鼠严重联合免疫缺陷（scid/scid）胎儿的研究表明，有核母体细胞经胎盘运输的频率很高。母体细胞在妊娠第 12.5 天 (gd) 首次出现在胸腺中，并且存在于 90% 以上的妊娠晚期胎儿中。形态异质的母体细胞主要位于骨髓和胸腺中，偶尔也位于肝、脾和非淋巴器官中。我们现在已经评估了淋巴系统发育正常的后代的母体细胞嵌合现象。将来自随机繁殖的 CD1 小鼠的遗传正常囊胚转移至 C57BL/6J-lacZ 转基因标记的 ROSA26 雌性小鼠中。对胎儿进行连续切片，然后对表达 lacZ 的细胞进行组织化学，用于全面定义含有母体细胞的器官。对胎儿进行整体切片，在 gd 16.5 之前没有检测到母体细胞。形态均质的有核母体细胞在 gd 16.5 时首次出现在胎儿骨髓腔中，并且在妊娠后期的所有后代中都很明显。出生后，通过 lacZ 组织化学和母体转基因的 PCR 扩增确定，母体细胞在成年期也存在于骨髓腔中。与妊娠后期相比，出生后骨髓中母源细胞的频率增加，偶尔在出生后脾脏中检测到母源细胞。母体细胞转移到具有遗传免疫能力的后代及其长期植入的正常情况表明母体细胞在后代中发挥功能作用。
Studies of murine severe combined immune-deficient (scid/scid) fetuses gestating in transgene-tagged immune competent dams have established high frequencies of transplacental trafficking of nucleated maternal cells. Maternal cells first appeared in thymus at gestation day (gd) 12.5 and were present in more than 90% of late gestation fetuses. Morphologically heterogeneous maternal cells were located predominantly in bone marrow and thymus and also occasionally in liver, spleen, and nonlymphoid organs. We have now evaluated maternal cell chimerism in offspring with normal lymphoid development. Genetically normal blastocysts from random-bred CD1 mice were transferred to C57BL/6J- lacZ transgene-tagged ROSA26 females. Serial sectioning of fetuses followed by histochemistry for lacZ-expressing cells was used to comprehensively define organs containing maternal cells. Fetuses, sectioned in their entirety, had no detectable maternal cells before gd 16.5. Morphologically homogenous, nucleated maternal cells were first present in fetal bone marrow cavities at gd 16.5 and were evident in all offspring in later gestation. Postnatally, maternal cells were also present in bone marrow cavities into adulthood, as determined by lacZ histochemistry and PCR amplification of the maternal transgene. The frequency of maternally derived cells in postnatal bone marrow was increased compared with late gestation, and occasionally, maternal cells were detected in postnatal spleen. The normalcy of maternal cell transfer to genetically immune competent progeny and their long-term engraftment is suggestive of a functional role for maternal cells in offspring.
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使用 T 细胞培养技术确定严重联合免疫缺陷 (SCID) 患者体内是否存在宫内来源的母体 T 细胞移植物。
影响因子：
6.2
作者：
Flomenberg,N;Dupont,B;O'Reilly,RJ;Hayward,A;Pollack,MS
通讯作者：
Pollack,MS
严重联合免疫缺陷患者移植母体 T 细胞的表型和功能。
DOI：
--
发表时间：
1984
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Thompson,LF;O'Connor,RD;Bastian,JF
通讯作者：
Bastian,JF
影响因子：
10.5
作者：
FRIEDRICH, G;SORIANO, P
通讯作者：
SORIANO, P
两名严重联合免疫缺陷患者的 XX T 细胞和 XY B 细胞。
DOI：
10.1016/0090-1229(84)90192-2
发表时间：
1984
期刊：
Clinical immunology and immunopathology
影响因子：
--
作者：
Conley,ME;Nowell,PC;Henle,G;Douglas,SD
通讯作者：
Douglas,SD
影响因子：
20.3
作者：
Evans, PC;Lambert, N;Nelson, JL
通讯作者：
Nelson, JL