Characterization of the complete mitochondrial genome of Bombyx mori strain H9 (Lepidoptera: Bombycidae)
Characterization of the complete mitochondrial genome of Bombyx mori strain H9 (Lepidoptera: Bombycidae)
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DOI：
10.1016/j.gene.2013.02.002
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发表时间：
2013-05-01
期刊：
影响因子：
3.5
通讯作者：
Liu, Chao-Liang
中科院分区：
文献类型：
--
作者：
Dai, Li-Shang;Zhu, Bao-Jian;Liu, Chao-Liang
家蚕品系 H9（鳞翅目：家蚕科）完整的线粒体基因组（线粒体基因组）长度为 15,670 个碱基对 (bp)，编码 13 个蛋白质编码基因 (PCG)、2 个 rRNA 基因、22 个 tRNA 基因和一个控制区。基因组核苷酸组成高度A+T偏态，占81.31%，AT偏度稍为正（0.059）。 13个PCG的排列与其他已测序的鳞翅目动物相似。所有 PCG 均由 ATN 密码子启动，但细胞色素 c 氧化酶亚基 I (cox1) 基因除外，该基因是由在其他鳞翅目动物中观察到的 TTAG 序列提出的。与其他 PCG 不同，cox1 和细胞色素 c 氧化酶亚基 2 (cox2) 基因具有仅由 T 组成的不完整终止密码子。所有 tRNA 均具有昆虫线粒体 tRNA 的典型结构，这与其他已测序的鳞翅目动物不同。富含A+T的区域的结构与其他已测序的鳞翅目动物的结构相似，包括非重复序列、ATAGA结合域、转移RNA M (trnM)基因上游的18bp聚T延伸和聚A元件。系统发育分析表明，家养蚕蛾B. mori起源于中国家蚕。 (C) 2013 Elsevier B.V. 保留所有权利。
The complete mitochondrial genome (mitogenome) of Bombyx mori strain H9 (Lepidoptera: Bombycidae) is 15,670 base pairs (bp) in length, encoding 13 protein-coding genes (PCGs), two rRNA genes, 22 tRNA genes and a control region. The nucleotide composition of the genome is highly A + T biased, accounting for 81.31%, with a slightly positive AT skewness (0.059). The arrangement of 13 PCGs is similar to that of other sequenced lepidopterans. All the PCGs are initiated by ATN codons, except for the cytochrome c oxidase subunit I (cox1) gene, which is proposed by the TTAG sequence as observed in other lepidopterans. Unlike the other PCGs, the cox1 and cytochrome c oxidase subunit 2 (cox2) genes have incomplete stop codons consisting of just a T. All tRNAs have typical structures of insect mitochondrial tRNAs, which is different from other sequenced lepidopterans. The structure of A + T-rich region is similar to that of other sequenced lepidopterans, including non-repetitive sequences, the ATAGA binding domain, a 18 bp poly-T stretch and a poly-A element upstream of transfer RNA M (trnM) gene. Phylogenetic analysis shows that the domesticated silkmoth B. mori originated from the Chinese Bombyx mandarina. (C) 2013 Elsevier B.V. All rights reserved.