CRYOPRESERVATION OF ZEBRA FISH SPERMATOZOA USING METHANOL
CRYOPRESERVATION OF ZEBRA FISH SPERMATOZOA USING METHANOL
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DOI：
10.1139/z82-242
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发表时间：
1982-01-01
影响因子：
1.4
通讯作者：
ASHWOODSMITH, MJ
中科院分区：
文献类型：
--
作者：
HARVEY, B;KELLEY, RN;ASHWOODSMITH, MJ
本发明描述了一种用于冷冻操作来自个体B的精液的方法。rerio使用甲醇和奶粉作为冷冻保护剂。活动性与孵化率呈正相关，平均为51 ± 1.5。35.6%，在一个典型的实验中。活力和孵化的变异与精子体积或年龄的鱼，并被认为是由于个体之间的精子质量的差异，以及解冻精子的活力持续时间短的技术限制。
A method is described for cryoperservation of milt from individual B. rerio using methanol and powdered milk as cryoprotectants. Motility was positively correlated with hatching, which averaged 51 .+-. 35.6% in a typical experiment. Variability in motility and hatching was not correlated with sperm volume or age of the fish, and is believed to be due to differences in sperm quality between individuals, as well as technical constraints imposed by the short duration of motility in thawed spermatozoa.