PROPERTIES OF A MOUSE RIBOSOMAL-PROTEIN PROMOTER
PROPERTIES OF A MOUSE RIBOSOMAL-PROTEIN PROMOTER
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DOI：
10.1073/pnas.83.22.8545
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发表时间：
1986-11-01
影响因子：
11.1
通讯作者：
PERRY, RP
中科院分区：
文献类型：
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作者：
DUDOV, KP;PERRY, RP
迄今为止已经分析的三种小鼠核糖体蛋白基因，尽管在进化上不相关，但在它们的5“末端显示出一些非常相似的序列特征，其中一些是由“管家”类别中的其他基因共享的。为了理解这些特征的意义，我们已经检查了编码核糖体蛋白L32和S16的基因以及一组L32基因的5“-缺失突变体在转染的灵长类细胞中的瞬时表达。我们的研究结果揭示了积极调节L32转录的元件的两个重要特征：它们不延伸超过36个核苷酸的主要转录起始位点的上游，并且它们对顺式连接的猿猴病毒40增强子元件的存在不敏感。管家基因启动子的一般设计特点，这些发现的相关性进行了讨论。
The three mouse ribosomal protein genes that have been analyzed to date, although evolutionarily unrelated, exhibit some remarkably similar sequence features at their 5'' ends, some of which are shared by other genes in the "house-keeping" category. In order to appreciate the significance of these features, we have examined the transient expression of the genes coding ribosomal proteins L32 and S16, as well as that of a set of 5''-deletion mutants of the L32 gene, in transfected primate cells. Our results reveal two important characteristics of the elements that positively regulate L32 transcription: they do not extend more than 36 nucleotides upstream of the major transcriptional initiation site, and they are insensitive to the presence of cis-linked simian virus 40 enhancer elements. The relevance of these findings to the general design features of housekeeping gene promoters is discussed.