Blotched snakehead virus is a new aquatic birnavirus that is slightly more related to avibirnavirus than to aquabirnavirus
Blotched snakehead virus is a new aquatic birnavirus that is slightly more related to avibirnavirus than to aquabirnavirus
复制标题
DOI：
10.1128/jvi.77.1.719-725.2003
复制
发表时间：
2003-01-01
影响因子：
5.4
通讯作者：
Delmas, B
中科院分区：
文献类型：
--
作者：
Da Costa, B;Soignier, S;Delmas, B
通过不同的方法，我们的特点，双RNA病毒斑点黑鱼病毒（BSNV）。基因组片段A的序列揭示了两个开放阅读框（ORF）的存在：具有3，207 bp长的核苷酸序列的大ORF和位于大ORF的N-末端一半内的417个核苷酸长的小ORF，但是在不同的阅读框中。发现大的ORF编码多蛋白，该多蛋白由病毒蛋白酶VP 4协同加工以产生pVP 2（VP 2前体）、71个氨基酸长的肽（[X]）、VP 4和VP 3。通过N-末端测序鉴定[X]-VP 4和VP 4-VP 3连接处的两个切割位点。我们表明，pVP 2的加工产生了VP 2和几个小肽（氨基酸[aa] 418至460，461至467，468至474和475至486）。这些肽中的两种（aa 418至460和475至486）在病毒颗粒中被阳性鉴定，另外10种肽来自肽aa 418至460的进一步加工。结果表明，VP 4切割多个Pro-X-Ala I Ala基序，但VP 4-VP 3连接处明显例外。预测的VP 4催化二联体的成员（Ser-692和Lys-729）的替换证实了它们在多蛋白加工中的不可或缺性。基因组片段B序列揭示了编码推定聚合酶VP 1的单个大ORF。我们的研究结果表明，BSNV应被认为是一个新的水生双RNA病毒物种，稍微更接近IBDV比IPNV。
By different approaches, we characterized the birnavirus blotched snakehead virus (BSNV). The sequence of genomic segment A revealed the presence of two open reading frames (ORFs): a large ORF with a 3,207-bp-long nucleotide sequence and a 417-nucleotide-long small ORF located within the N-terminal half of the large ORF, but in a different reading frame. The large ORF was found to encode a polyprotein cotranslationally processed by the viral protease VP4 to generate pVP2 (the VP2 precursor), a 71-amino-acid-long peptide ([X]), VP4, and VP3. The two cleavage sites at the [X]-VP4 and VP4-VP3 junctions were identified by N-terminal sequencing. We showed that the processing of pVP2 generated VP2 and several small peptides (amino acids [aa] 418 to 460, 461 to 467, 468 to 474, and 475 to 486). Two of these peptides (aa 418 to 460 and 475 to 486) were positively identified in the viral particles with 10 additional peptides derived from further processing of the peptide aa 418 to 460. The results suggest that VP4 cleaves multiple Pro-X-Ala I Ala motifs, with the notable exception of the VP4-VP3 junction. Replacement of the members of the predicted VP4 catalytic dyad (Ser-692 and Lys-729) confirmed their indispensability in the polyprotein processing. The genomic segment B sequence revealed a single large ORF encoding a putative polymerase, VP1. Our results demonstrate that BSNV should be considered a new aquatic birnavirus species, slightly more related to IBDV than to IPNV.