Microsatellite typing identifies the major clades of the human pathogen Candida albicans
Microsatellite typing identifies the major clades of the human pathogen Candida albicans
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DOI：
10.1016/j.meegid.2010.03.007
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发表时间：
2010-07-01
影响因子：
3.2
通讯作者：
Sampaio, Paula
中科院分区：
文献类型：
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作者：
Chavez-Galarza, Julio;Pais, Celia;Sampaio, Paula
白色念珠菌种群研究表明，该物种可以分为密切相关的菌株，由分支指定的亚组。自微卫星分析作为一种基于PCR的方法出现以来，该技术已成功地用作区分C.白色念珠菌分离株，但从未测试过五个主要分支的聚类。在本研究中，我们检测了微卫星长度多态性（MLP）对29 C群的影响.先前定义为属于进化枝I、II、III、E和SA的白色念珠菌菌株，来自安哥拉和马达加斯加的9种非典型菌株，以及78种葡萄牙临床分离株。MLP分型的116个菌株分析产生了87个不同的多位点等位基因组合，导致在一个高的判别力指数为0.987，只有两个标记，CA1和CEF3。聚类分析的29个先前定义的菌株分组，根据他们的分支名称与矩阵同表型相关性r = 0.963后，归一化曼特尔统计。聚类分析的116株保持相同的分组，明确界定了五个主要的白色念珠菌分支。获得的同表型值为r = 0.839，1000个随机排列中归一化Mantel统计量的单尾概率为P = 0.0020。Ⅰ、Ⅱ、Ⅲ和SA组葡萄牙分离株所占比例分别为2.7%、15.4%、3.8%和0%。所有分离株均未与非典型菌株共聚。这些结果证实MLP分型是一种很好的分型和鉴别C.白色念珠菌分离株和组分离株，确定主要的白色念珠菌分支，类似于Ca3指纹和多位点序列分型（MLST）。(C)2010 Elsevier B.V.保留所有权利。
Candida albicans population studies showed that this species could be divided into sub-groups of closely related strains, designated by clades. Since the emergence of microsatellite analysis as a PCR based method, this technique has been successfully used as a tool to differentiate C. albicans isolates but has never been tested regarding clustering of the five major clades. In this study we tested microsatellite length polymorphism (MLP) ability to group 29 C. albicans strains previously defined as belonging to clades I, II, Ill, E and SA, nine atypical strains from Angola and Madagascar, and 78 Portuguese clinical isolates. MLP typing of the total 116 strains analyzed yielded 87 different multilocus allelic combinations which resulted in a high discriminatory power index, of 0.987, with only two markers, CA1 and CEF3. Cluster analysis of the 29 previously defined strains grouped them according to their clade designation with a matrix cophenetic correlation of r = 0.963 after a normalized Mantel statistic. Clustering analysis of the 116 strains maintained the same groupings, clearly defining the five major C albicans clades. The cophenetic value obtained was of r = 0.839, and the one-tail probability of the normalized Mantel statistic out of 1000 random permutations was P = 0.0020. The proportion of Portuguese isolates in the groups I, II, III and SA was of 2.7%, 15.4%, 3.8% and 0%, respectively. None of the isolates co-clustered with the atypical strains. These results confirm MLP typing as a good method both to type and differentiate C. albicans isolates and to group isolates, identifying the major C albicans clades, similarly to Ca3 fingerprinting and multilocus sequence typing (MLST). (C) 2010 Elsevier B.V. All rights reserved.