IMAGING ADHESION FORCES AND ELASTICITY OF LYSOZYME ADSORBED ON MICA WITH THE ATOMIC-FORCE MICROSCOPE
IMAGING ADHESION FORCES AND ELASTICITY OF LYSOZYME ADSORBED ON MICA WITH THE ATOMIC-FORCE MICROSCOPE
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DOI：
10.1021/la00022a068
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发表时间：
1994-10-01
期刊：
影响因子：
3.9
通讯作者：
HANSMA, PK
中科院分区：
文献类型：
--
作者：
RADMACHER, M;FRITZ, M;HANSMA, PK
利用原子力显微镜（AFM）研究了缓冲溶液中溶菌酶分子在云母表面的吸附。吸附物的稳定性受缓冲条件的影响。在pH 4时，单个分子可以稳定地成像，而在pH 6.4时，仅观察到分子的聚集体。通过记录横向分辨力曲线获得尖端-样品相互作用的地图。这些力曲线表明，尖端在溶菌酶上的粘附力小于在云母上的粘附力。通过对这些力曲线的分析，可以计算出溶菌酶的本征粘弹特性。
We have investigated the adsorption of lysozyme molecules on mica substrates in buffer by atomic force microscopy (AFM). The stability of the adsorbate is influenced by buffer conditions. At pH 4 single molecules can be imaged stably, while at pH 6.4 only aggregates of molecules are observed. Maps of the tip-sample interaction were obtained by recording laterally resolved force curves. These force curves showed that the adhesion of the tip on the lysozyme is smaller than on the mica. The intrinsic viscoelastic properties of lysozyme could be calculated by analyzing these force curves.