MAIZE PLASTID GENE EXPRESSED DURING PHOTOREGULATED DEVELOPMENT
MAIZE PLASTID GENE EXPRESSED DURING PHOTOREGULATED DEVELOPMENT
复制标题
DOI：
10.1073/pnas.75.7.3060
复制
发表时间：
1978-01-01
影响因子：
11.1
通讯作者：
RICH, A
中科院分区：
文献类型：
--
作者：
BEDBROOK, JR;LINK, G;RICH, A
从暗生长的玉米幼苗的病质体和光诱导发育后期的质体中制备出大于6 S的rna，在体外进行标记，并将其与内切酶酶切的玉米叶绿体DNA分离片段杂交。主要的非核糖体RNA存在于发育中的质体中，但在病变质体中几乎无法检测到，它与叶绿体dna片段8杂交，并已被定位在玉米质体染色体上。来自质体的其他等分RNA在兔网织细胞衍生系统中被翻译。发育中的质体和成熟的叶绿体，但不包括病变体，含有约34500道尔顿多肽的mRNA。在光诱导的玉米质体发育过程中，与toam8杂交并翻译成34500道尔顿蛋白的RNA同时出现，表明正在观察到单个基因的光调控表达。
RNAs larger than about 6 S prepared from etioplasts of dark-grown maize seedlings, and from plastids at later stages of light-induced development, were labeledin vitroand hybridized to separated fragments of maize chloroplast DNA digested with endonucleases. The major nonribosomal RNA present in developing plastids, but virtually undetectable in etioplasts, hybridizes to chloroplast DNABamfragment 8 and has been mapped on the maize plastid chromosome. Other aliquots of RNA from plastids were translated in a rabbit reticulocyte-derived system. Developing plastids, and mature chloroplasts, but not etioplasts, contain mRNA for an approximately 34,500 dalton polypeptide. The simultaneous appearance, during light-induced maize plastid development, of RNA which hybridizes toBam8 and is translated into a 34,500 dalton protein indicates that photoregulated expression of a single gene is being observed.