Limitation of a localized surface plasmon resonance sensor for Salmonella detection
Limitation of a localized surface plasmon resonance sensor for Salmonella detection
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DOI：
10.1016/j.snb.2009.06.020
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发表时间：
2009-08-18
影响因子：
8.4
通讯作者：
Zhao, Yiping
中科院分区：
文献类型：
--
作者：
Fu, Junxue;Park, Bosoon;Zhao, Yiping
我们设计了一种局域表面等离子体共振(LSPR)生物传感器，利用斜角沉积技术制备的金纳米颗粒用于沙门氏菌的全细胞检测。扫描电子显微镜和荧光显微镜证实，由于沙门氏菌抗原和抗沙门氏菌抗体的反应，LSPR传感器的等离子体峰发生了移动。然而，这种变化对细菌的浓度并不敏感。我们利用Mie理论和有效介质理论对该检测系统进行了建模，发现由于纳米粒子与细菌的接触面积较小以及局域电场的短程相互作用，无论细菌的浓度如何，该系统引起的等离子体激元峰位移都在2-4 nm左右。这与实验结果是一致的，如果将LSPR传感器用于全细胞细菌检测，则需要另一种方法。(C)2009爱思唯尔B.V.保留所有权利。
We have designed a localized surface plasmon resonance (LSPR) biosensor to perform whole cell detection of Salmonella using Au nanoparticles fabricated by an oblique angle deposition technique. The LSPR sensor shows a plasmon peak shift due to the Salmonella antigen and anti-Salmonella antibody reaction as verified by scanning electron microscopy and fluorescence microscopy. However, this shift is not sensitive to the concentration of the bacteria. We have modeled this detection system by means of Mie theory and effective medium theory, and find that due to the small contact area between the nanoparticle and the bacteria and the short range interaction of the local electric field, the plasmon peak shift induced by such a system is about 2-4 nm, regardless of the concentration of the bacteria. This is consistent with the experiments, and an alternative method is needed if the LSPR sensor is used for whole cell bacteria detection. (C) 2009 Elsevier B.V. All rights reserved.