Mbp1, a component of the MluI cell cycle box-binding complex, contributes to morphological transition and virulence in the filamentous entomopathogenic fungus Beauveria bassiana
Mbp1, a component of the MluI cell cycle box-binding complex, contributes to morphological transition and virulence in the filamentous entomopathogenic fungus Beauveria bassiana
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Mbp1 是 MluI 细胞周期盒结合复合物的一个组成部分，有助于丝状昆虫病原真菌白僵菌的形态转变和毒力
DOI：
10.1111/1462-2920.14868
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发表时间：
2019-12-02
影响因子：
5.1
通讯作者：
Ying, Sheng-Hua
中科院分区：
文献类型：
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作者：
Ding, Jin-Li;Lin, Hai-Yan;Ying, Sheng-Hua
Mbp1蛋白在MluI细胞周期盒结合复合物中作为DNA结合蛋白发挥功能，并在酵母发育中发挥重要作用。在这项研究中，直系同源的酵母Mbp1，BbMbp1，其特征在于在丝状昆虫真菌，球孢白僵菌。BbMbp1在空气和液体环境下的形态变化中起重要作用。在气生表面，BbMbp1是分生孢子梗和分生孢子发生所必需的。在淹没条件下，增量BbMbp1突变体显示出异常的产孢子结构，与芽生孢子产量急剧下降（类似于95%）。BbMbp1突变体的毒力明显减弱，这可能是由于在昆虫体内形成芽生孢子的缺陷。此外，破坏BbMbp1导致尸体上菌丝生长的大幅减少。比较转录组学研究表明，BbMbp1在分生孢子和芽生孢子形成过程中介导不同的转录组。酵母单杂交试验表明，BbMbp1是所需的转录控制细胞壁蛋白基因，BbCwp，和一个完整的膜蛋白基因，BbImp在分生孢子和芽生孢子形成分别发挥重要作用。我们的研究结果表明，BbMbp1有助于形态转变的致病性和恶性生长的B。通过不同的遗传途径。
The Mbp1 protein functions as a DNA-binding protein in the MluI cell cycle box-binding complex and plays significant roles in yeast development. In this study, an ortholog of yeast Mbp1, BbMbp1, was characterized in a filamentous insect mycopathogen, Beauveria bassiana. BbMbp1 plays an important role in morphological changes under aerial and liquid environments. On the aerial surface, BbMbp1 was indispensable for the biogenesis of conidiophores and conidiation. Under submerged conditions, the increment BbMbp1 mutant displayed abnormal spore-producing structures, with a dramatic decrease in blastospore yield (similar to 95%). The virulence of the increment BbMbp1 mutant was notably weakened, which might be due to the defect in in vivo blastospore formation in the insect. Moreover, disruption of BbMbp1 resulted in a substantial reduction in hyphal growth on cadavers. Comparative transcriptomics revealed that BbMbp1 mediated different transcriptomes during the formation processes of conidia and blastospores. Yeast one-hybrid assays demonstrated that BbMbp1 was required for transcriptional control of a cell wall protein gene, BbCwp, and an integral membrane protein gene, BbImp that played significant roles in conidiation and blastospore formation respectively. Our results demonstrate that BbMbp1 contributes to the morphological transitions in the pathogenic and saprophytic growth of B. bassiana via different genetic pathways.