Tungsten-enhanced growth of Methanosphaera stadtmanae.
Tungsten-enhanced growth of Methanosphaera stadtmanae.
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DOI：
10.1186/1756-0500-5-238
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发表时间：
2012-05-15
影响因子：
1.8
通讯作者：
Drancourt M
中科院分区：
文献类型：
--
作者：
Dridi B;Khelaifia S;Fardeau ML;Ollivier B;Drancourt M
已通过培养和非培养方法在人类肠道微生物群中检测到产甲烷菌Methanosphaera stadtmanae。在322培养基（10%vol）中培养5 - 7天后，其生长达到指数生长期。我们最近成功分离到一种需要钨酸盐和亚硒酸盐的嗜酸菌，它与甲烷微球菌具有相似的代谢特征。stadtmanae提示我们研究钨和硒对M.城市增长向培养基322中加入0.2mg/L钨酸钠，在接种后48小时，通过甲烷监测和光密度测量得到的生长速率与对照培养物6天后得到的生长速率相当。添加50 μg/mL硒酸钠对M.城市增长定量实时PCR靶向M。Stadtmanae 16 S rRNA证实了这些数据。这些数据提供了新的信息，关于鲜为人知的营养需求的人类肠道殖民organismsM。城市。在基础培养基中加入钨酸钠可以促进这种生物的表型表征，并有助于从复杂的宿主微生物群中分离新的微生物。
The methanogenic Archaea Methanosphaera stadtmanae has been detected in the human gut microbiota by both culture and culture-independent methods. Its growth reaches an exponential phase after 5 to 7-day culture in medium 322 (10% vol). Our recent successful isolation of Methanomassiliicoccus luminyensis, a tungstate-selenite-requiring Archaea sharing similar metabolism characteristics with M. stadtmanae prompted us to study the effects of tungsten and selenium on M. stadtmanae growth. Addition of 0.2 mg/L sodium tungstate to medium 322 yielded, 48 hours after inoculation, a growth rate equivalent to that obtained after 6 days with control culture as measured by methane monitoring and optical density measurement. Addition of 50 μg/mL sodium selenate had no effect on M. stadtmanae growth. Quantitative real-time PCRs targeting the M. stadtmanae 16S rRNA confirmed these data. These data provide new information regarding the poorly known nutritional requirements of the human gut colonizing organismsM. stadtmanae. Adding sodium tungstate to basal medium may facilitate phenotypic characterization of this organism and additionally aid the isolation of new Archaeafrom complex host microbiota.