Order-of-magnitude multiphoton signal enhancement based on characterization of absorption spectra of immersion oils at the 1700-nm window
Order-of-magnitude multiphoton signal enhancement based on characterization of absorption spectra of immersion oils at the 1700-nm window
复制标题
基于 1700 nm 窗口浸油吸收光谱表征的数量级多光子信号增强
DOI：
10.1364/oe.25.005909
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发表时间：
2017-03-20
期刊：
影响因子：
3.8
通讯作者：
Qiu, Ping
中科院分区：
文献类型：
--
作者：
Wang, Ke;Wen, Wenhui;Qiu, Ping
为了提高油浸多光子显微镜（MPM）在深层组织激发窗口（1600-1820 nm）的信号水平，我们证明了：首先，表征了几种常用浸泡油的吸收光谱，这是以前未知的。其次，提出了基于混合的低吸收新材料。第三，基于吸收光谱表征，提出了该窗口内激发波长的最佳选择。小鼠组织的二次和三次谐波成像证实了我们的选择：与1700 nm激发相比，1600 nm激发导致MPM信号显著增加，使小鼠皮肤的成像深度达到200 μ m，而1700 nm激发几乎无法分辨任何结构。(C) 2017年美国光学学会
To enhance signal levels in multiphoton microscopy (MPM) at the deep-tissue excitation window (1600-1820 nm) with oil immersion, we demonstrate: First, the absorption spectra of several commonly immersion oils are characterized, which were unknown before. Second, new material with lower absorption based on mixing is proposed. Third, optimal selection of excitation wavelength within this window is proposed based on absorption spectra characterization. Second and third harmonic generation imaging of mouse tissue corroborate our selection: 1600-nm excitation leads to notable orders-of-magnitude increase in MPM signal, compared with 1700-nm excitation, enabling 200-mu m imaging depth of mouse skin while 1700-nm excitation could resolve virtually no structure. (C) 2017 Optical Society of America