Role of alveolar macrophages in initiation and regulation of inflammation in Pseudomonas aeruginosa pneumonia
Role of alveolar macrophages in initiation and regulation of inflammation in Pseudomonas aeruginosa pneumonia
复制标题
DOI：
10.1128/iai.66.7.3164-3169.1998
复制
发表时间：
1998-07-01
影响因子：
3.1
通讯作者：
Sawa, T
中科院分区：
文献类型：
--
作者：
Kooguchi, K;Hashimoto, S;Sawa, T
为探讨肺泡巨噬细胞（AM）在小鼠急性铜绿假单胞菌肺炎中的作用，通过雾化吸入含氯膦酸二钠的脂质体清除AM。然后用5 × 10（5）CFU的铜绿假单胞菌对AM耗尽的小鼠进行气管内感染。除了监测中性粒细胞募集和趋化因子释放外，还在感染后不久（8小时）和稍后时间（48小时）评价肺损伤。在8小时，AM的消耗减少了中性粒细胞募集、趋化因子释放和肺损伤。然而，在48 h时，AM的消耗降低了细菌清除率，并导致中性粒细胞从炎症部位的运动延迟，肺损伤加重。与非AM耗竭小鼠相比，在注入5 × 10（7）CFU细菌后，AM耗竭小鼠在感染后24小时内死亡率较低，但在随后的时间内死亡率较高。这些结果表明，在铜绿假单胞菌肺炎的情况下，AM的消耗对肺损伤和存活具有有益的早期效应，但具有有害的晚期效应。
To evaluate the role of alveolar macrophages (AMs) in acute Pseudomonas aeruginosa pneumonia in mice, AMs were depleted by aerosol inhalation of liposomes containing clodronate disodium. AM-depleted mice,were then intratracheally infected with 5 x 10(5) CFU of P, aeruginosa. In addition to monitoring neutrophil recruitment and chemokine releases, lung injury was evaluated soon after infection (8 h) and at a later time (48 h), At 8 h, depletion of AMs reduced neutrophil recruitment, chemokine release, and lung injury. At 48 h, however, depletion of AMs decreased bacterial clearance and resulted in delayed movement of neutrophils from the site of inflammation with aggravated lung injury. With instillation of 5 x 10(7) CFU of bacteria, AM-depleted mice showed low mortality within 24 h of infection but high mortality at a later time, in contrast to non-AM-depleted mice. These results demonstrate that depletion of AMs has beneficial early effects but deleterious late effects on lung injury and survival in cases of P. aeruginosa pneumonia.