Transcriptome Profiling of the Salt-Stress Response in the Halophytic Green Alga Dunaliella salina
Transcriptome Profiling of the Salt-Stress Response in the Halophytic Green Alga Dunaliella salina
复制标题
盐生绿藻杜氏盐胁迫响应的转录组分析
DOI：
10.1007/s11105-019-01168-z
复制
发表时间：
2019-10-25
影响因子：
2.1
通讯作者：
Ji, Zhiyong
中科院分区：
文献类型：
--
作者：
Li, Liang;Zhang, Xiaochai;Ji, Zhiyong
盐藻细胞在高盐环境下会发生形态、生长、色素等方面的变化以适应胁迫。尽管D。盐藻细胞适应盐环境，极端高盐度通过释放活性氧，提高相对电导率，对细胞构成威胁。为了生存，D。盐沼保存了大量的甘油，以防止高盐度的危害。为了揭示盐胁迫下耐盐的分子基础，本研究利用转录组测序技术对盐胁迫下的盐胁迫相关基因进行了鉴定。salina。基于多数据库分析，共鉴定和注释了40，682个unigenes。我们发现，与低盐条件相比，约717-1012个unigenes在高盐条件下上调或下调。研究结果表明，盐胁迫上调了光合作用和甘油代谢的关键基因，本研究是迄今为止最大规模的转录组D的比较研究。盐藻能适应高盐环境，为进一步研究藻类和高等植物耐盐的分子机制奠定了基础。
Due to the hypersaline environment cell of Dunaliella salina can change its morphology, growth, and pigment for adapting to the stress. Despite the fact D. salina cell was accustomed to the salt environment, extreme high salinity threatened cell by releasing reactive oxygen species, which raise relative electrical conductivity. With the purpose of surviving, D. salina saved plenty of glycerol to prevent the harm of high salinity. To find out the molecular basis for salinity tolerance, transcriptome sequencing was used to identify salt-regulated genes in D. salina. A total of 40,682 unigenes were identified and annotated based on the multiple databases analysis. We found out that about 717–1012 unigenes are up- or downregulated in hypersaline compared to hyposaline condition. The results of research showed that salinity stress upregulated key genes in photosynthesis and glycerol metabolism, this research is the largest comparative investigation of transcriptome D. salina adapted to very salinity environment, creating a solid base for analyzing the molecular mechanism of salt tolerance of algae and higher plants.