THE DEMONSTRATION BY RIBOTYPING OF THE STABILITY OF ORAL STREPTOCOCCUS-MUTANS INFECTION OVER 5 TO 7 YEARS IN CHILDREN
THE DEMONSTRATION BY RIBOTYPING OF THE STABILITY OF ORAL STREPTOCOCCUS-MUTANS INFECTION OVER 5 TO 7 YEARS IN CHILDREN
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DOI：
10.1016/0003-9969(94)90142-2
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发表时间：
1994-06-01
影响因子：
3
通讯作者：
ASIKAINEN, S
中科院分区：
文献类型：
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作者：
ALALUUSUA, S;ALALUUSUA, SJ;ASIKAINEN, S
调查了7名5岁、10岁和12岁儿童分离的变形链球菌的血清型和核糖型分布。对于核糖体分型，用限制性内切酶EcoRI和HindIII对每个受试者5-13个分离物的染色体DNA进行酶切。DNA片段经电泳法分离，印迹到尼龙膜上，与含有大肠杆菌染色体rRNA操纵子的pKK3535载体杂交。每个孩子的核型都是独一无二的。在5名儿童中，仅发现一种核糖型和血清型(c、e或f)。在一名儿童中，在基线时发现了两种血清型(c和f)，在随访样本中只发现了一种(c型)。在一名儿童中，在基线(血清型e)和随访(血清型c)样本中未发现相同的血清型。除一名儿童外，所有儿童的核糖体类型与5-7岁后发现的核糖体类型相同。结果表明，在5岁时，变形链球菌的感染已经稳定下来，定植菌株仍然是永久性的。
The distribution of serotypes and ribotypes of mutans streptococcal isolates obtained from seven unrelated children at 5 and at 10 or 12 yr of age was investigated. For ribotyping, chromosomal DNA from 5 to 13 isolates per subject was digested with restriction endonucleases EcoRI and HindIII. The DNA fragments were electrophoretically separated, blotted on to nylon membrane and hybridized to the plasmid pKK3535, which contains the rRNA operon of the Escherichia coli chromosome. The ribotypes were unique for each child. In five children only one ribotype and serotype (c, e or f) was found. In one child two serotypes (c and f) were found at baseline and only one (serotype c) in the follow-up sample. In one child the same serotype was not found in the baseline (serotype e) and in the follow-up (serotype c) samples. Every child except one had a ribotype that was identical to one found 5-7 yr later. The results suggest that, at the age of 5 yr, infection by Streptococcus mutans has already stabilized and the colonizing strain remains permanent.