Immobilization of Sertoli cells on Islets of Langerhans
Immobilization of Sertoli cells on Islets of Langerhans
复制标题
支持细胞在朗格汉斯岛上的固定化
DOI：
10.1039/c2bm00048b
复制
发表时间：
2013
影响因子：
6.6
通讯作者：
Hiroo Iwata
中科院分区：
文献类型：
--
作者：
Naohiro Takenoio;YUji Termura;Hiroo Iwata
支持细胞在创造睾丸的免疫保护环境中起着至关重要的作用。我们研究了与支持细胞共移植后胰岛的存活情况。胰岛附近的支持细胞应保护移植物免受排斥反应。在这项研究中，单链寡核苷酸，聚（乙二醇）和磷脂（ssDNA-PEG-DPPE）的共轭物被用来在胰岛上的支持细胞。20-mer的脱氧腺苷酸（oligo（dA）20）和20-mer的脱氧胸苷酸（oligo（dT）20）作为ssDNAs的支持细胞和胰岛的表面上，分别通过之间的疏水相互作用的结合物的脂质单元和细胞膜。通过oligo（dA）20和oligo（dT）20之间的杂交将Sertoli细胞固定在胰岛上。当Sertoli细胞固定的胰岛通过门静脉输注到小鼠肝脏中时，Sertoli细胞仍保留在胰岛周围。
Sertoli cells play a crucial role in creating the immunoprivileged environment of the testis. We examined the survival of islets of Langerhans after co-transplantation with Sertoli cells. Sertoli cells near islets should protect the graft from rejection. In this study, conjugates of single stranded oligonucleotides, poly(ethylene glycol) and phospholipids (ssDNA-PEG-DPPE) were used to immobilize Sertoli cells on islets. The 20-mer of deoxyadenylic acid (oligo(dA)20) and 20-mer of deoxythymidylic acid (oligo(dT)20) were presented as ssDNAs on the surfaces of Sertoli cells and islets, respectively, through the hydrophobic interaction between a lipid unit of the conjugates and the cell membrane. The Sertoli cells were immobilized on the islets through hybridization between oligo(dA)20 and oligo(dT)20. When Sertoli cell-immobilized islets were infused into the liver of mice through the portal vein, the Sertoli cells remained around the islets.