Swine centromeric DNA repeats revealed by primed in situ (PRINS) labeling
Swine centromeric DNA repeats revealed by primed in situ (PRINS) labeling
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DOI：
10.1159/000134687
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发表时间：
1997-01-01
期刊：
影响因子：
--
通讯作者：
Vaiman, M
中科院分区：
文献类型：
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作者：
Rogel-Gaillard, C;Hayes, H;Vaiman, M
在猪中，已经描述了不同的着丝粒卫星DNA家族，它们要么对应于除Y(Mc1)以外的所有中着丝粒染色体，要么对应于所有的顶着丝粒染色体(Ac2)。利用原位引物标记，我们在这里展示了来自不同序列的引物特异性地标记不同染色体亚群的着丝粒。在报道的5个来自着丝粒染色体着丝粒序列的引物中，有4个能识别所有的着丝粒染色体，1个能识别17号染色体。对于中着丝粒染色体，有6个引物是从几个不同的序列中衍生出来的。在这些引物中，有两个能识别除5、10和12以外的所有中着丝粒染色体。另外三个标记中着丝粒染色体的小亚群，包括X和一个或两个额外的染色体。最后一条是针对1号染色体的。这些初步结果表明，应该有可能为几乎每条猪染色体定义特定的引物。这里报道的一些引物已经允许区分不带就很难区分的猪染色体，如X染色体和9号染色体。因此，使用着丝粒基序的Prins技术构成了猪细胞遗传学研究的另一种工具。
In swine, distinct centromeric satellite DNA families have been described that correspond to either all the metacentric chromosomes except the Y (Mc1) or all the acrocentric chromosomes (Ac2). Using primed in situ (PRINS) labeling, we show here that primers derived from various sequences specifically label the centromeres of different subgroups of chromosomes. Among five primers derived from centromeric sequences of acrocentric chromosomes reported to be very homogeneous, four recognize all the acrocentric chromosomes, whereas one labels prominently chromosome 17. For the metacentric chromosomes, six primers have been derived from several divergent sequences. Among these primers, two recognize all the metacentric chromosomes except 5, 10, and 12. Three other primers label small subsets of metacentric chromosomes, including the X and one or two additional chromosomes. The last primer is specific to chromosome 1. These preliminary results suggest that it should be possible to define specific primers for almost every swine chromosome. Already, some of the primers reported here permit a distinction between swine chromosomes difficult to differentiate without banding, such as the X chromosome and chromosome 9. Therefore, the PRINS technique using centromeric motifs constitutes an additional tool for cytogenetic studies in swine.