CARS microscopy using linearly chirped ultrafast laser pulses
CARS microscopy using linearly chirped ultrafast laser pulses
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使用线性啁啾超快激光脉冲的 CARS 显微镜
DOI：
10.1117/12.809411
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
Rocha-Mendoza I
中科院分区：
文献类型：
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作者：
Rocha-Mendoza I
我们已经开发了一个自制的汽车显微镜，利用线性啁啾超快激光脉冲。通过使用高群速度色散的玻璃，150 fs持续时间的傅里叶限制的斯托克斯和泵浦脉冲被同样啁啾到0.5 ps-2.8 ps范围内的脉冲持续时间。通过这种方式，我们将瞬时频率差的频谱宽度减小到啁啾脉冲持续时间的傅立叶极限（频谱聚焦）。作为原理的证明，在聚苯乙烯珠上展示了具有高光谱分辨率的汽车光谱。我们还表明，无论是理论和实验，啁啾脉冲的持续时间短于或相当于拉曼相干时间，最大汽车信号发生在斯托克斯脉冲后到达的泵。此外，我们通过对不同的活细胞和固定的组织样品进行汽车显微光谱分析，证明了我们的CARS显微镜在生物科学中的适用性。
We have developed a home-built CARS microscope which exploits linearly-chirped ultrafast laser pulses. By using glass of high group-velocity dispersion, Stokes and Pump pulses of 150 fs duration Fourier-limited are equally chirped to pulse durations in the 0.5 ps-2.8 ps range. In this way we reduce the spectral width of the instantaneous frequency difference to the Fourier limit of the chirped pulse duration (spectral focussing). As a proof of principle, CARS spectroscopy with high spectral resolution is demonstrated on polystyrene beads. We also show, both theoretically and experimentally, that for chirped pulse durations shorter than or comparable to the Raman coherence time, maximum CARS signal occurs for a Pump arriving after the Stokes pulse. Furthermore, we demonstrate the applicability of our CARS microscope to biological sciences by performing CARS microspectroscopy on different live cells and fixed tissue samples.