Ectopic Expression of Carpel-Specific MADS Box Genes from Lily and Lisianthus Causes Similar Homeotic Conversion of Sepal and Petal in Arabidopsis1
Ectopic Expression of Carpel-Specific MADS Box Genes from Lily and Lisianthus Causes Similar Homeotic Conversion of Sepal and Petal in Arabidopsis1
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DOI：
10.1104/pp.007948
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发表时间：
2002-12
期刊：
影响因子：
7.4
通讯作者：
T. Tzeng;Hsing-Yu Chen;Chang-Hsien Yang
中科院分区：
文献类型：
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作者：
T. Tzeng;Hsing-Yu Chen;Chang-Hsien Yang
两个MADS盒基因，百合MADS盒基因2（LMADS 2）和洋桔梗MADS盒基因1（EgMADS 1），与矮牵牛花结合蛋白7/11和拟南芥AGL 11具有广泛的相似性，其特征在于来自百合（Lilium longiflorum）和洋桔梗（Eustoma grandiflorum）。LMADS 2和EgMADS 1 mRNA仅在心皮中表达，在其它花器官和营养叶中不表达。LMADS 2 mRNA主要在胚珠中表达，心皮花柱组织中表达较弱，而EgMADS 1 mRNA仅在胚珠中表达。异位表达LMADS 2或EgMADS 1的转基因拟南芥植株表现出与35 S：：AGAMOUS植株相似的新表型，表现为植株大小显著减小，开花早，花序不确定性消失。这两个基因的异位表达也产生了类似的ap 2样花诱导同源异型转化的萼片心皮样结构，其中柱头乳头和胚珠观察。此外，在35 S：：LMADS 2和35 S：：EgMADS 1转基因拟南芥的第二轮中，花瓣转化为雄蕊样结构。我们的数据表明，LMADS 2和EgMADS 1是百合和洋桔梗中推测的D功能MADS盒基因，其功能类似于拟南芥中异位表达的C功能基因。
Two MADS box genes, Lily MADS Box Gene 2(LMADS2) and Eustoma grandiflorum MADS Box Gene 1 (EgMADS1), with an extensive similarity to the petunia (Petunia hybrida) FLORAL BINDING PROTEIN 7/11 and Arabidopsis AGL11, were characterized from the lily (Lilium longiflorum) and lisianthus (Eustoma grandiflorum). The expression ofLMADS2 and EgMADS1 mRNA was restricted to the carpel and was absent in the other flower organs or vegetative leaves. LMADS2 mRNA was detected mainly in ovules and weakly in style tissues of the carpel, whereas EgMADS1mRNA was only expressed in the ovules. Transgenic Arabidopsis plants ectopically expressing LMADS2 or EgMADS1showed similar novel phenotypes resembling35S::AGAMOUS plants by significantly reducing plant size, flowering early, and losing inflorescence indeterminacy. Ectopic expression of these two genes also generated similar ap2-like flowers by inducing homeotic conversion of the sepals into carpel-like structures in which stigmatic papillae and ovules were observed. In addition, the petals were converted into stamen-like structures in the second whorl of 35S::LMADS2 and35S::EgMADS1 transgenic Arabidopsis. Our data indicated that LMADS2 andEgMADS1 are putative D functional MADS box genes in lily and lisianthus with a function similar to C functional genes once ectopically expressed in Arabidopsis.