Multi-omics provide mechanistic insight into the Pb-induced changes in tadpole fitness-related traits and environmental water quality.
Multi-omics provide mechanistic insight into the Pb-induced changes in tadpole fitness-related traits and environmental water quality.
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DOI：
10.1016/j.ecoenv.2022.114207
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发表时间：
2022-10
影响因子：
6.8
通讯作者：
Yan Lv;Qun-De Zhang;Liming Chang;Duoli Yang;Luqman Riaz;Cheng Li;Xiao-Hong Chen;Jian-Ping Jiang-Jian
中科院分区：
文献类型：
--
作者：
Yan Lv;Qun-De Zhang;Liming Chang;Duoli Yang;Luqman Riaz;Cheng Li;Xiao-Hong Chen;Jian-Ping Jiang-Jian
铅/Pb2+水污染对水生生态系统构成了重大威胁，其对水生动物的影响已引起广泛关注。虽然已发现Pb2+影响动物的许多方面，包括个体适应性，代谢状态和共生菌群，但很少有研究关注Pb2+诱导的适应性，代谢组，共生菌群和同一系统中的环境参数变化之间的关联，限制了从整体角度对生态毒理学机制的全面理解。此外，大多数生态毒理学研究忽视了阴离子对无机铅化合物产生的后果的潜在贡献。以空白对照组和NaNO3处理组为对照，研究了环境相关浓度的Pb（NO3）2对林蛙蝌蚪及其周围水质的影响。结果表明，Pb（NO3）2不仅会导致水中亚硝酸盐水平的升高，而且暴露于这种化学物质还会损害蝌蚪健康相关的性状（例如，成长与发展）。对蝌蚪的影响最有可能是Pb2+和NO3-的组合。组织代谢组学显示，Pb（NO3）2暴露影响动物基质（即，碳水化合物、脂质和氨基酸）和前列腺素代谢。Pb（NO3）2在肠道微生物群中产生了深刻的变化，变形菌的增加削弱了厚壁菌门，导致更高的需氧菌和可能的致病菌。NaNO3也影响蝌蚪代谢组和肠道微生物组，以不同的方式Pb（NO3）2。NO3-的存在似乎抵消了Pb2+引起的一些变化，特别是对微生物群的影响。分段结构方程模型和相关性分析表明组织代谢组和肠道微生物组之间存在联系，蝌蚪表型性状和水质的变化与组织代谢组和肠道微生物组的变化有关。这些发现强调了肠道微生物组在介导毒素对水生态系统的影响中的重要作用。建议在重金属污染风险评价中考虑阴离子的影响。
Water pollution from lead/Pb2+poses a significant threat to aquatic ecosystems, and its repercussions on aquatic animals have received considerable attention. Although Pb2+has been found to affect numerous aspects of animals, including individual fitness, metabolic status, and symbiotic microbiota, few studies have focused on the associations between Pb2+-induced variations in fitness, metabolome, symbiotic microbiome, and environmental parameters in the same system, limiting a comprehensive understanding of ecotoxicological mechanisms from a holistic perspective. Moreover, most ecotoxicological studies neglected the potential contributions of anions to the consequences generated by inorganic lead compounds. We investigated the effects of Pb(NO3)2at environmentally relevant concentrations on theRana omeimontistadpoles and the water quality around them, using blank and NaNO3-treated groups as control. Results showed that Pb(NO3)2not only induced a rise in water nitrite level, but exposure to this chemical also impaired tadpole fitness-related traits (e.g., growth and development). The impacts on tadpoles were most likely a combination of Pb2+and NO3-. Tissue metabolomics revealed that Pb(NO3)2exposure influenced animal substrate (i.e., carbohydrate, lipid, and amino acid) and prostaglandin metabolism. Pb(NO3)2produced profound shifts in gut microbiota, with increased Proteobacteria impairing Firmicutes, resulting in higher aerobic and possibly pathogenic bacteria. NaNO3also influenced tadpole metabolome and gut microbiome, in a manner different to that of Pb(NO3)2. The presence of NO3-seemed to counteract some changes caused by Pb2+, particularly on the microbiota. Piecewise structural equation model and correlation analyses demonstrated connections between tissue metabolome and gut microbiome, and the variations in tadpole phenotypic traits and water quality were linked to changes in tissue metabolome and gut microbiome. These findings emphasized the important roles of gut microbiome in mediating the effects of toxin on aquatic ecosystem. Moreover, it is suggested to consider the influences of anions in the risk assessment of heavy metal pollutions.