A Novel Genotype of Hepatitis E Virus Prevalent Among Farmed Rabbits in China
A Novel Genotype of Hepatitis E Virus Prevalent Among Farmed Rabbits in China
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DOI：
10.1002/jmv.21536
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发表时间：
2009-08-01
影响因子：
12.7
通讯作者：
Wang, Youchun
中科院分区：
文献类型：
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作者：
Zhao, Chenyan;Ma, Zhongren;Wang, Youchun
从中国甘肃省的两个养殖场共采集了335份家兔血清样本，采用EIA法检测戊型肝炎病毒（HEV）抗体，采用ORF2引物嵌套式RTPCR法检测HEV RNA。抗HEV抗体和HEV RNA总阳性率分别为57.0%（191/335）和7.5%（25/335）。抗HEV抗体阴性组的HEV RNA阳性率（7.6%(11/144)）与阳性组（7.3%(14/191)）无显著差异。HEV RNA与抗HEV抗体的一致性为43.3%，相关性不显著（P < 0.05）。对ORF2区125个扩增子进行克隆和测序。核苷酸序列比较表明，它们与1、2、3、4和禽HEV基因型对应区域的同源性分别为84-99%、73-77%、70-76%、75-82%、71-77%和53-65%。ORF2引物阳性的样品用ORR区引物扩增；在核苷酸水平上，17个基因型阳性，分别与1、2、3、4和禽HEV基因型对应区域的同源性分别为71-78%、73-76%、74-82%、72-78%和39-58%。确定了两个具有代表性的全长序列。这两个序列与1、2、3、4和禽HEV全长基因型的同源性分别为74%、73%、78-79%、74-75%和46-47%。因此，从家兔中分离的序列代表了一种新的HEV基因型。这项研究提供了关于兔感染HEV基因型的新信息，以及一种新的哺乳动物HEV基因型的证据。中华医学杂志，2009,31(1):379 - 379。(C) 2009 Wiley-Liss, Inc。
In total, 335 serum samples were collected from rabbits from two farms in Gansu province, China, and tested for anti-hepatitis E virus (HEV) antibody using EIA and for HEV RNA using nested RTPCR with ORF2 primers. The overall prevalence of anti-HEV antibody and HEV RNA was 57.0% (191/335) and 7.5% (25/335), respectively. The positivity rate of HEV RNA in the anti-HEV antibody negative group (7.6% (11/144)) did not differ significantly from that in the positive group (7.3% (14/191)). The concordance between HEV RNA and anti-HEV antibody was 43.3% with no significant correlation (P < 0.05). All 125 amplicons from the ORF2 region were cloned and sequenced. On the basis of nucleotide sequence comparison, they had 84-99% identity to each other and 73-77%, 70-76%, 75-82%, 71-77%, and 53-65% with the corresponding regions of genotypes 1, 2, 3, 4, and avian HEV, respectively. Samples that were positive with the ORF2 primers were amplified using ORR region primers; 17 were positive and shared 71-78%, 73-76%, 74-82%, 72-78%, and 39-58% identity with the corresponding regions of genotypes 1, 2, 3, 4, and avian HEV, respectively, at the nucleotide level. Two representative full-length sequences were determined. These two sequences shared 85% identity with each other and had 74%, 73%, 78-79%, 74-75%, and 46-47% identity to full-length genotypes 1, 2, 3, 4, and avian HEV, respectively. Thus, the sequences isolated from the rabbits represent a novel genotype of HEV. This study provides novel information about HEV genotypes infecting rabbits as well as evidence of a new mammalian genotype of HEV. J. Med. Virol. 81:137-11379, 2009. (C) 2009 Wiley-Liss, Inc.