Oxidant and antioxidant profile of hyperketonemic ewes affected by pregnancy toxemia
Oxidant and antioxidant profile of hyperketonemic ewes affected by pregnancy toxemia
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DOI：
10.1111/j.1939-165x.2011.00284.x
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发表时间：
2011-03-01
影响因子：
1.2
通讯作者：
Al-Qudah, Khaled M.
中科院分区：
文献类型：
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作者：
Al-Qudah, Khaled M.
研究背景母羊妊娠后期能量负平衡是引起高酮血症的重要原因。酮体可以产生超氧自由基，引起氧化应激和细胞功能障碍，如在患有亚临床酮病的奶牛或糖尿病患者中所指出的。本研究的目的是评估高酮血症在启动脂质过氧化过程中的作用。（年龄3.5-6岁），10只临床健康的妊娠母羊和10只临床健康的非妊娠母羊。测定血清β-羟基丁酸酯（BHB）、皮质醇和葡萄糖浓度，谷胱甘肽过氧化物酶、超氧化物歧化酶和过氧化氢酶的血浆活性，硫代巴比妥酸反应物质（TBARS）、脂质过氧化标记物和还原型谷胱甘肽的血浆浓度。结果妊娠毒血症母羊血清BHB、皮质醇和TBARS浓度显著高于正常妊娠组和非妊娠组（P
BackgroundNegative energy balance during late pregnancy in ewes is an important cause of hyperketonemia. Ketone bodies can generate superoxide radicals and cause oxidative stress and cellular dysfunction, as noted in cows with subclinical ketosis or in diabetic people.ObjectiveThe aim of this study was to evaluate the role of hyperketonemia in initiating the process of lipid peroxidation.MethodsThe study included 10 pregnant ewes (aged 3.5-6 years) with pregnancy toxemia, 10 clinically healthy pregnant ewes, and 10 clinically healthy nonpregnant ewes. Serum concentrations of beta-hydroxybutyrate (BHB), cortisol, and glucose, plasma activities of glutathione peroxidase, superoxide dismutase, and catalase, and plasma concentrations of thiobarbituric acid reactive substances (TBARS), markers of lipid peroxidation, and reduced glutathione were measured. Data from the 3 groups were statistically analyzed and compared.ResultsSerum concentrations of BHB, cortisol, and TBARS were significantly higher in ewes with pregnancy toxemia when compared with concentrations in healthy pregnant and nonpregnant groups (P