Improvement of analysis throughput for LC/MS assay
Improvement of analysis throughput for LC/MS assay
复制标题
提高 LC/MS 测定的分析通量
DOI：
10.1007/s44211-022-00089-4
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
S. Ogawa
中科院分区：
文献类型：
--
作者：
Takeru Sagae;Mariko Yokogawa;Ryoichi Sawazaki;Yuichiro Ishii;Nao Hosoda;Shin-ichi Hoshino;Shunsuke Imai;Ichio Shimada;Masanori Osawa;S. Ogawa
分析各种生物样品中的化合物，包括体液，组织，细胞，甚至是食品原料或食品成分，可以帮助我们了解它们的生物活性，诊断各种疾病，发现新的药物和新的生物标志物。液相色谱（LC）/质谱（MS或MS/MS）是代谢物定量的最强大和最可靠的方法，因为它的选择性和灵敏度，以及许多化合物的广泛覆盖。众所周知，化学衍生方法通常使LC/MS的检测能力更强，因为衍生代谢物具有理想的LC分离行为和增强的MS测定灵敏度[1-3]。Ohno等人报道了一种新型质子亲合衍生试剂：5-丁基吡啶甲酸的开发，并将该试剂应用于唾液中痕量睾酮和二氢睾酮的分析[4]。此外，Nagatomo等人，将衍生化方法应用于LC/MS/MS法测定粪便样品中的短链脂肪酸[5]。如上述示例所示，在LC/MS/MS分析中使用衍生化作为灵敏度提高方法的报道仍在继续[6-8]。另一方面，LC/MS分析需要提高分析通量。因为LC/MS方法每次分析需要较长的测量时间，以换取色谱分离测量值的高可靠性。因此，衍生化也可能是克服这一缺点的一种有希望的方法。即在单次LC/MS运行中对一些分析样品进行多路复用，这是通过使用多种试剂对多个样品进行衍生化来实现的
Analysis of compounds in various biological samples, including body fluids, tissues, cells, and even raw food material or food ingredients, may contribute our understanding of their biological activities, diagnosis for various diseases, new drug and novel biomarker discovery. Liquid chromatography (LC)/mass spectrometry (MS or MS/MS) is the most powerful and reliable methodology for the quantification of metabolites due to its selectivity and sensitivity, and broad coverage of many compounds. As is already widely known, chemical derivatization method often makes the detection ability of LC/MS more powerfully due to the desirable LC separation behavior and enhanced MS assay sensitivity of the derivatized metabolites [1–3]. Ohno et al. reported a development of novel proton affinitive derivatization reagent: 5-butylpicolinic acid and applied the reagent to the analysis of a trace amount of testosterone and dihydrotestosterone in saliva [4]. Furthermore, Nagatomo et al., applied the derivatization method to determination of short-chain fatty acids in feces samples by LC/MS/MS [5]. As shown in the above example, the use of derivatization as a sensitivity improvement method in LC/MS/MS analysis continues to be reported [6–8].On the other hand, LC/MS assays have been required improvements in analytical throughput. Because the LC/MS method requires a long measurement time for each analysis in exchange for the high reliability of the measured values by chromatographic separation. Thus, the derivatization might be also a hopeful approach to overcome this disadvantage. That is the multiplexing of some analytical samples in the single LC/MS run, which is achieved by derivatization of the multiple samples with the multiple reagents