Constructing multispecies biofilms with defined compositions by sequential deposition of bacteria
Constructing multispecies biofilms with defined compositions by sequential deposition of bacteria
复制标题
通过细菌的顺序沉积构建具有确定成分的多物种生物膜
DOI：
10.1007/s00253-010-2473-y
复制
发表时间：
2010
影响因子：
5
通讯作者：
E. Gilbert
中科院分区：
文献类型：
--
作者：
B. Stubblefield;Kristen E. Howery;B. Islam;Ariel J. Santiago;Wendy E. Cardenas;E. Gilbert
合理组合的多物种生物膜可将互补的代谢途径组合成单一的功能群落，从而有利于包括混合废物生物降解和药物生物合成在内的应用过程。我们假设，成熟的多物种生物膜的细胞组成可以通过控制新定居表面上存在的每种细胞类型的数量来操纵。为了测试这一想法，我们开发了一种方法，通过循环细胞悬浮液将特定数量的细菌附着到流动细胞上。最初的工作显示，当两个菌株同时再循环时，悬浮细胞密度和面密度之间存在非线性关系；相反，顺序再循环导致可预测的细胞数量沉积。对由大肠杆菌菌株组成的48h生物膜中细胞分布的定量分析表明，它们在基质中的分布与它们在成熟生物膜中的存在有很强的相关性。用铜绿假单胞菌或枯草芽孢杆菌按顺序沉积大肠杆菌，确定了再循环的第二种微生物的面密度与其单独再循环时的面密度之间的微小但可重复的差异。总体而言，该方法提供了一种简单且可重复的方法来构建具有特定组成的多物种生物膜，用于生物催化过程。
Rationally-assembled multispecies biofilms could benefit applied processes including mixed waste biodegradation and drug biosynthesis by combining complementary metabolic pathways into single functional communities. We hypothesized that the cellular composition of mature multispecies biofilms could be manipulated by controlling the number of each cell type present on newly colonized surfaces. To test this idea, we developed a method for attaching specific numbers of bacteria to a flow cell by recirculating cell suspensions. Initial work revealed a nonlinear relationship between suspension cell density and areal density when two strains of Escherichia coli were simultaneously recirculated; in contrast, sequential recirculation resulted in a predictable deposition of cell numbers. Quantitative analysis of cell distributions in 48-h biofilms comprised of the E. coli strains demonstrated a strong relationship between their distribution at the substratum and their presence in mature biofilms. Sequentially depositing E. coli with either Pseudomonas aeruginosa or Bacillus subtilis determined small but reproducible differences in the areal density of the second microorganism recirculated relative to its areal density when recirculated alone. Overall, the presented method offers a simple and reproducible way to construct multispecies biofilms with defined compositions for biocatalytic processes.