The use of surface-enhanced Raman scattering for detecting molecular evidence of life in rocks, sediments, and sedimentary deposits.
The use of surface-enhanced Raman scattering for detecting molecular evidence of life in rocks, sediments, and sedimentary deposits.
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使用表面增强拉曼散射来检测岩石、沉积物和沉积物中生命的分子证据。
DOI：
10.1089/ast.2009.0435
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发表时间：
2010
期刊：
影响因子：
4.2
通讯作者：
J. Parnell
中科院分区：
文献类型：
--
作者：
S. Bowden;Rab Wilson;J. Cooper;J. Parnell
拉曼光谱是一种通用的分析技术，能够表征无机和有机材料的成分。因此，它经常被提议作为许多行星着陆器的有效载荷。大约每10⁶个光子中只有1个发生拉曼散射；因此，检测分散在样品基质中的痕量分析物可能要困难得多。为了克服这一问题，表面增强拉曼散射（SERS）和表面增强共振拉曼散射（SERRS）都通过分析物与局部产生的表面等离子体的相互作用提供了大幅增强的信号（增强倍数在10⁵到10⁹之间），这种表面等离子体出现在“粗糙”或纳米结构的金属表面（例如，铜、金和银）。因此，SERS和SERRS都可能为样品的痕量分析提供一种可行的技术。在本文中，我们描述了用于分析沉积矿床溶剂提取物中痕量化合物的SERS检测方法的开发。这些检测方法被用于检测北极微绿洲（一个生物生产力较高的小区域）及其碎屑风化层中存在的生物色素，表征热液泉周围微生物席的色素沉着，并检测热液矿床中的化石有机物。这些实地研究实例表明，SERS技术已经足够成熟，可以应用于地球上的许多天体生物学模拟研究。许多当前和拟议的用于远程部署的成像系统已经具备SERS所需的仪器组件。在这些仪器中增加湿化学样品处理设施，可以产生可在实地部署的分析仪器，其分析窗口更宽，可用于检测具有生物或地质来源的有机化合物。
Raman spectroscopy is a versatile analytical technique capable of characterizing the composition of both inorganic and organic materials. Consequently, it is frequently suggested as a payload on many planetary landers. Only approximately 1 in every 10(6) photons are Raman scattered; therefore, the detection of trace quantities of an analyte dispersed in a sample matrix can be much harder to achieve. To overcome this, surface-enhanced Raman scattering (SERS) and surface-enhanced resonance Raman scattering (SERRS) both provide greatly enhanced signals (enhancements between 10(5) and 10(9)) through the analyte's interaction with the locally generated surface plasmons, which occur at a "roughened" or nanostructured metallic surface (e.g., Cu, Au, and Ag). Both SERS and SERRS may therefore provide a viable technique for trace analysis of samples. In this paper, we describe the development of SERS assays for analyzing trace amounts of compounds present in the solvent extracts of sedimentary deposits. These assays were used to detect biological pigments present in an Arctic microoasis (a small locale of elevated biological productivity) and its detrital regolith, characterize the pigmentation of microbial mats around hydrothermal springs, and detect fossil organic matter in hydrothermal deposits. These field study examples demonstrate that SERS technology is sufficiently mature to be applied to many astrobiological analog studies on Earth. Many current and proposed imaging systems intended for remote deployment already posses the instrumental components needed for SERS. The addition of wet chemistry sample processing facilities to these instruments could yield field-deployable analytical instruments with a broadened analytical window for detecting organic compounds with a biological or geological origin.
体外痤疮丙酸杆菌脂质水解的表面增强拉曼光谱监测。
影响因子：
6.5
作者：
Weldon,MK;Morris,MD;Harris,AB;Stoll,JK
通讯作者：
Stoll,JK
影响因子：
2.9
作者：
Chi, ZH;Chen, XG;Asher, SA
通讯作者：
Asher, SA