Genes for murine fourth complement component (C4) and sex-limited protein (Slp) identified by hybridization to C4- and Slp-specific cDNA.
Genes for murine fourth complement component (C4) and sex-limited protein (Slp) identified by hybridization to C4- and Slp-specific cDNA.
复制标题
通过与 C4 和 Slp 特异性 cDNA 杂交鉴定出鼠第四补体成分 (C4) 和性别限制蛋白 (Slp) 的基因。
DOI：
10.1073/pnas.81.15.4908
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发表时间：
1984
影响因子：
11.1
通讯作者：
Sepich,DS
中科院分区：
文献类型：
--
作者：
Ogata,RT;Sepich,DS
小鼠补体第四成分(C4)和性限蛋白(SLP)是两种密切相关的血清蛋白，其结构基因位于小鼠H-2复合体的S区域。我们已经克隆了两个非常相似的(95%的核苷酸序列同源性)cDNA；它们编码的氨基酸序列是不同的，但与小鼠和人类C4可用的有限氨基酸序列一样好。在RNA印迹杂交实验中，我们通过比较这两个cDNA与近交系小鼠体内表达不同数量的C4和SLP蛋白的肝脏mRNAs的杂交程度，鉴定了其中一个为C4基因，另一个为SLP基因。利用C4和SLP基因片段进行Southern杂交，以确定S地区分子图谱中的C4和SLP基因。
Murine fourth component of complement (C4) and sex-limited protein (Slp) are two closely related serum proteins whose structural genes lie in the S region of the murine H-2 complex. We have cloned two very similar (95% nucleotide sequence identity) cDNAs; they encode amino acid sequences that are distinct but that correspond equally well with the limited amino acid sequence available for murine and human C4. We have identified one of these as C4 cDNA and the other as Slp cDNA by comparing, in a RNA blot hybridization experiment, the extent of hybridization of the two cDNAs to liver mRNAs from inbred mouse strains expressing varying amounts of C4 and Slp proteins in their plasma. The C4 and Slp cDNAs were used in a Southern blot experiment to identify the C4 and Slp genes in the molecular map of the S region.