The effect of extracellular matrix interactions on morphologic transformation in vitro.
The effect of extracellular matrix interactions on morphologic transformation in vitro.
复制标题
细胞外基质相互作用对体外形态转化的影响。
DOI：
10.1016/0006-291x(91)91374-l
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发表时间：
1991
影响因子：
3.1
通讯作者：
Coffey,DS
中科院分区：
文献类型：
--
作者：
Pienta,KJ;Murphy,BC;Getzenberg,RH;Coffey,DS
越来越多的证据表明，细胞的结构和功能在一定程度上取决于细胞与细胞外基质的接触。本文报道了正常和肿瘤细胞分泌的细胞外基质对正常大鼠肾上皮细胞结构的影响。正常大鼠肾细胞接种于肿瘤细胞分泌的基底膜上，其形态和表型与Kirsten-ras转化的正常大鼠肾细胞极为相似。在单个细胞外基质成分或正常胎盘细胞分泌的基底膜上，细胞没有观察到这种形态变化。提示肿瘤来源的基底膜具有独特的特性，可能引起正常大鼠肾细胞的形态转化。
There is emerging evidence that the structure and function of a cell is dependent in part on the contacts that cells make with the extracellular matrix. We report here the effect of extracellular matrices secreted from both normal and tumor cells have on the structure of normal rat kidney epithelial cells. Normal rat kidney cells plated on the basement membrane secreted by tumor cells adopt a morphology and phenotype which closely resembles a Kirsten-ras transformed normal rat kidney cell. This morphologic transformation was not observed for cells plated on individual extracellular matrix components or on basement membrane secreted by normal placenta cells. This suggests that tumor derived basement membrane has unique characteristics which may cause morphologic transformation of normal rat kidney cells.