Compensatory change of interacting amino acids in the coevolution of transcriptional coactivator MBF1 and TATA-box-binding protein
Compensatory change of interacting amino acids in the coevolution of transcriptional coactivator MBF1 and TATA-box-binding protein
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DOI：
10.1093/molbev/msm073
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发表时间：
2007-07-01
影响因子：
10.7
通讯作者：
Gojobori, Takashi
中科院分区：
文献类型：
--
作者：
Liu, Qing-Xin;Nakashima-Kamimura, Naomi;Gojobori, Takashi
了解转录因子、转录辅激活因子和TATA盒结合蛋白（TATA-box-binding protein，TBP）的协同进化，对于阐明真核生物基因组网络中的转录调控具有重要意义。在这项研究中，共同进化的转录辅激活多蛋白桥接因子1和它的相互作用的目标TBP的第一次实验评估，通过检查是否补偿氨基酸的变化发生在相互作用的网站，这两种蛋白质。通过确定相互作用位点并比较不同生物体之间这些位点的氨基酸进行实验。在这里，我们提供了证据的转录辅激活因子及其相互作用的靶点的补偿性变化，首次报告转录辅激活因子可能与TBP经历了协同进化。
To elucidate the transcriptional regulation in eukaryotic genome network, it is important to understand coevolution of transcription factors, transcriptional coactivators, and TATA-box-binding protein (TBP). In this study, coevolution of transcriptional coactivator multiprotein-bridging factor 1 and its interacting target TBP was first evaluated experimentally by examining if compensatory amino acid changes took place at interacting sites of both proteins. The experiments were conducted by identifying interaction sites and comparing the amino acids at these sites among different organisms. Here, we provide evidence for compensatory changes of transcription coactivator and its interacting target, presenting the 1st report that transcription coactivator may have undergone coevolution with TBP.