A simplified sample pretreatment for the rapid determination of 22 β-agonist residues in swine muscle and liver tissues by ultra-high-performance liquid chromatography tandem mass spectrometry
A simplified sample pretreatment for the rapid determination of 22 β-agonist residues in swine muscle and liver tissues by ultra-high-performance liquid chromatography tandem mass spectrometry
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超高效液相色谱串联质谱法快速测定猪肌肉和肝脏组织中 22 β-激动剂残留的简化样品预处理
DOI：
10.1016/j.jchromb.2018.07.038
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发表时间：
2018-10-01
影响因子：
3
通讯作者：
Wang, Dong
中科院分区：
文献类型：
--
作者：
Guo, Ping;Wan, Jianchun;Wang, Dong
建立了一种可靠的超高效液相色谱-串联质谱（UHPLC-MS/MS）同时鉴定和定量猪肌肉和肝脏组织中22种β激动剂残留的方法。特别关注样品的预处理程序，这对于准确可靠的分析方法至关重要。针对食品基质的高复杂性，对萃取溶剂、萃取时间、萃取温度以及清洗筒进行优化，以提高萃取效率，降低基质效应。通过线性（R-2 >= 0.9900）、灵敏度（检测限为0.05 ~ 0.8 μ g/kg，定量限为0.2 ~ 2.5 μ g/kg）、回收率（76.4 ~ 111.7%）和精密度(
A reliable ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method was developed for the simultaneous identification and quantification of 22 beta-agonist residues in swine muscle and liver tissues. A special focus was given to sample the pretreatment procedures and was crucial for an accurate and reliable analytical method. With regard to the high complexity of food matrices, the extraction solvent, time, and temperature, as well as clean-up cartridges, were optimized to improve the extraction efficiency and reduce matrix effects. The UHPLC-MS/MS method was further validated by determining the linearity (R-2 >= 0.9900), sensitivity (limit of detection varying from 0.05 mu g/kg to 0.8 mu g/kg, limit of quantitation ranging from 0.2 mu g/kg to 2.5 mu g/kg), recovery (ranging from 76.4 to 111.7%) and precision (