DNA-SEQUENCE ANALYSIS OF A COMPLEMENTARY-DNA FOR COLD-REGULATED ARABIDOPSIS GENE COR15 AND CHARACTERIZATION OF THE COR-15 POLYPEPTIDE
DNA-SEQUENCE ANALYSIS OF A COMPLEMENTARY-DNA FOR COLD-REGULATED ARABIDOPSIS GENE COR15 AND CHARACTERIZATION OF THE COR-15 POLYPEPTIDE
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DOI：
10.1104/pp.99.2.519
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发表时间：
1992-06-01
期刊：
影响因子：
7.4
通讯作者：
THOMASHOW, MF
中科院分区：
文献类型：
--
作者：
LIN, CT;THOMASHOW, MF
以往的研究表明，基因表达的变化发生在拟南芥L。（Heyn），并且某些cor（冷调节）基因编码共享在水溶液中煮沸后保持可溶的不寻常性质的多肽。在这里，我们确定了一个cDNA克隆的冷调节基因编码的“煮沸稳定”的多肽，COR 15。DNA序列分析表明，该基因，命名为cor 15，编码一个14.7千道尔顿的亲水性多肽，具有一个N-末端氨基酸序列，非常类似于转运肽，目标蛋白质的叶绿体基质室。免疫学研究表明，COR 15是在体内加工，成熟的多肽，COR 15 m，存在于叶绿体的可溶性部分。讨论了COR 15 m的可能功能。
Previous studies have indicated that changes in gene expression occur in Arabidopsis thaliana L. (Heyn) during cold acclimation and that certain of the cor (cold-regulated) genes encode polypeptides that share the unusual property of remaining soluble upon boiling in aqueous solution. Here, we identify a cDNA clone for a cold-regulated gene encoding one of the "boiling-stable" polypeptides, COR15. DNA sequence analysis indicated that the gene, designated cor15, encodes a 14.7-kilodalton hydrophilic polypeptide having an N-terminal amino acid sequence that closely resembles transit peptides that target proteins to the stromal compartment of chloroplasts. Immunological studies indicated that COR15 is processed in vivo and that the mature polypeptide, COR15m, is present in the soluble fraction of chloroplasts. Possible functions of COR15m are discussed.