Refocused primary echo: A zero dead time detection of the electron spin echo envelope modulation
Refocused primary echo: A zero dead time detection of the electron spin echo envelope modulation
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DOI：
10.1006/jmre.1999.1988
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发表时间：
2000-04-01
影响因子：
2.2
通讯作者：
Raitsimring, AM
中科院分区：
文献类型：
--
作者：
Astashkin, AV;Raitsimring, AM
我们报道了再聚焦一次电子自旋回波包络调制（ESEEM）技术的二维（2D）实现、其理论以及在一个模型体系和一个具有生物学意义的体系中的实验应用。这种技术在一个时间坐标上几乎没有死时间。通过对再聚焦一次ESEEM的二维时域数据在其中一个时间坐标上进行积分所获得的ESEEM显示，对于k远小于1，和组合谐波的强度与k²成正比，对于k近似于1则与k成正比（k是调制幅度因子的常用符号）。这一特性，与通过改变光谱仪的工作频率来调节k相结合，被发现对于检测靠近电子自旋且具有分布超精细相互作用参数的质子非常有用。（C）2000学术出版社。
We report on the two-dimensional (2D) implementation of the refocused primary electron spin echo envelope modulation (ESEEM) technique, its theory and experimental application to a model system and a system of biological interest. This technique is virtually free of dead time along one time coordinate. The ESEEM obtained by integration of the 2D time-domain data of the refocused primary ESEEM over one of the time coordinates shows the intensity of the sum combination harmonics proportional to k(2) for k much less than 1 and proportional to k for k similar to 1 (k is a usual notation for the modulation amplitude factor). This feature, in combination with the adjustment of k by means of variation of the operational frequency of the spectrometer, was found to be very useful for detection of protons with distributed hyperfine interaction parameters situated close to the electron spin. (C) 2000 Academic Press.