Occludin as a functional marker of vascular endothelial cells on tube-forming activity
Occludin as a functional marker of vascular endothelial cells on tube-forming activity
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DOI：
10.1002/jcp.26082
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发表时间：
2018-02-01
影响因子：
5.6
通讯作者：
Yamaguchi, Teruhide
中科院分区：
文献类型：
--
作者：
Kanayasu-Toyoda, Toshie;Ishii-Watabe, Akiko;Yamaguchi, Teruhide
使用内皮祖细胞(EPC)进行细胞治疗是治疗缺血性疾病的一种有前途的策略。EPC有两种类型：早期EPC和晚期EPC。晚期内皮祖细胞能够自行形成管状结构，具有较高的增殖能力。晚期内皮祖细胞的功能标记物(S)与其治疗潜力有关，但尚未完全阐明。在这里，我们比较了几个具有不同管状形成活性的人脐带血来源的晚期EPC系的基因表达谱，我们观察到OCLN在这些系中的表达与管状形成能力有关，提示OCLN是晚期EPC的候选功能标记。当OCLN被siRNA敲除后，Matrigel上的小管形成、细胞周期中的S期+G(2)/M期和晚期内皮祖细胞的球状萌发明显减少，提示OCLN在小管的形成、萌发和增殖中起着关键作用。这些结果表明，OCLN在新生血管和血管生成中发挥了新的作用。
Cell therapy using endothelial progenitor cells (EPCs) is a promising strategy for the treatment of ischemic diseases. Two types of EPCs have been identified: early EPCs and late EPCs. Late EPCs are able to form tube structure by themselves, and have a high proliferative ability. The functional marker(s) of late EPCs, which relate to their therapeutic potential, have not been fully elucidated. Here we compared the gene expression profiles of several human cord blood derived late EPC lines which exhibit different tube formation activity, and we observed that the expression of occludin (OCLN) in these lines correlated with the tube formation ability, suggesting that OCLN is a candidate functional marker of late EPCs. When OCLN was knocked down by transfecting siRNA, the tube formation on Matrigel, the S phase+G(2)/M phase in the cell cycle, and the spheroid-based sprouting of late EPCs were markedly reduced, suggesting the critical role of OCLN in tube formation, sprouting, and proliferation. These results indicated that OCLN plays a novel role in neovascularization and angiogenesis.