Gas Plasma-Treated Prostate Cancer Cells Augment Myeloid Cell Activity and Cytotoxicity
Gas Plasma-Treated Prostate Cancer Cells Augment Myeloid Cell Activity and Cytotoxicity
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DOI：
10.3390/antiox9040323
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发表时间：
2020-04-01
期刊：
影响因子：
7
通讯作者：
Stope, Matthias B.
中科院分区：
文献类型：
--
作者：
Bekeschus, Sander;Ressel, Verena;Stope, Matthias B.
尽管最近癌症治疗有所改善，其中许多与培养抗肿瘤免疫有关，但与肿瘤相关的死亡率仍然很高。需要新的途径来补充现有的肿瘤治疗策略。医用气体等离子体技术近年来因其抗肿瘤作用而受到重视。气等离子体通过局部沉积过多的活性氧（ROS）来促进氧化性癌细胞死亡。然而，血浆介导的肿瘤细胞死亡的免疫学后果尚不清楚。为此，我们将两株前列腺癌细胞（LNCaP、PC3）体外暴露于气体血浆中，研究了其上清液与两株人髓系（THP-1、HL-60）直接共培养的免疫调节作用。在确定kINPen等离子射流的细胞毒性作用后，血浆处理的前列腺癌细胞的上清液调节髓细胞相关线粒体ROS的产生及其代谢活性、增殖、表面标记物表达和细胞因子释放。与未经处理的对照组相比，直接共培养可增强髓细胞中分化样表面标记物的表达，并提高其在气体血浆中的抗肿瘤毒性。结果表明，气浆源性活性氧不仅促进前列腺癌细胞死亡，而且增强骨髓细胞活性和细胞毒性。
Despite recent improvements in cancer treatment, with many of them being related to foster antitumor immunity, tumor-related deaths continue to be high. Novel avenues are needed to complement existing therapeutic strategies in oncology. Medical gas plasma technology recently gained attention due to its antitumor activity. Gas plasmas act via the local deposition of a plethora of reactive oxygen species (ROS) that promote the oxidative cancer cell death. The immunological consequences of plasma-mediated tumor cell death are only poorly understood, however. To this end, we exposed two prostate cancer cell lines (LNCaP, PC3) to gas plasma in vitro, and investigated the immunomodulatory effects of the supernatants in as well as of direct co-culturing with two human myeloid cell lines (THP-1, HL-60). After identifying the cytotoxic action of the kINPen plasma jet, the supernatants of plasma-treated prostate cancer cells modulated myeloid cell-related mitochondrial ROS production and their metabolic activity, proliferation, surface marker expression, and cytokine release. Direct co-culture amplified differentiation-like surface marker expression in myeloid cells and promoted their antitumor-toxicity in the gas plasma over the untreated control conditions. The results suggest that gas plasma-derived ROS not only promote prostate cancer cell death but also augment myeloid cell activity and cytotoxicity.