APPLICATION OF DYNAMIC CAPILLARY ISOELECTRIC-FOCUSING TO THE ANALYSIS OF HUMAN HEMOGLOBIN-VARIANTS
APPLICATION OF DYNAMIC CAPILLARY ISOELECTRIC-FOCUSING TO THE ANALYSIS OF HUMAN HEMOGLOBIN-VARIANTS
复制标题
DOI：
10.1002/elps.1150150105
复制
发表时间：
1994-01-01
期刊：
影响因子：
2.9
通讯作者：
THORMANN, W
中科院分区：
文献类型：
--
作者：
MOLTENI, S;FRISCHKNECHT, H;THORMANN, W
本文报道了正常和病理血红蛋白（Hb）的毛细管等电聚焦电泳（CIEF）。CIEF是在未经处理的，开放的管状，熔融石英毛细管75 μ m内径使用甲基纤维素动态柱条件。在直接注射与载体两性电解质混合的溶血产物后，获得了Hb变体的高分辨率分离，包括Hb A（1c）、A、F、D、S、E和A（2），这允许明确地表征正常成人、新生儿、糖尿病、不同血红蛋白病和地中海贫血综合征患者的Hb模式。定性地，CIEF数据与通过凝胶等电聚焦和高效液相色谱法获得的数据比较好。CIEF被证明是一种简单、快速和完全仪器化的Hb分析方法。运行时间不到20分钟，使CIEF成为常规血红蛋白研究和筛查项目的一种有吸引力的方法。
Capillary isoelectric focusing (CIEF) with electroosmotic zone displacement of normal and pathological hemoglobins (Hb) is reported. CIEF is performed in untreated, open-tubular, fused-silica capillaries of 75 mu m internal diameter using methylcellulose for dynamic column conditioning. After direct injection of hemolysates mixed with carrier ampholytes, high resolution separation of Hb variants, including Hb A(1c), A, F,D, S, E and A(2), is obtained, this permitting unambiguous characterization of Hb patterns of normal adults, newborns, patients with diabetes, different hemoglobinopathies and thalassemia syndromes. Qualitatively, the CIEF data compare well with those obtained by gel isoelectric focusing and high-performance liquid chromatography. CIEF is demonstrated to be a simple, rapid and fully instrumental approach to Hb analysis. Run times of less than 20 min make CIEF an attractive method for routine Hb investigations and screening programs.