Hypericum erectum alcoholic extract inhibits Toxoplasma growth and Entamoeba encystation: an exploratory study on the anti-protozoan potential.
Hypericum erectum alcoholic extract inhibits Toxoplasma growth and Entamoeba encystation: an exploratory study on the anti-protozoan potential.
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金丝桃醇提取物抑制弓形虫生长和内阿米巴包囊：抗原虫潜力的探索性研究。
DOI：
10.1007/s11418-019-01369-6
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Yoshida H.
中科院分区：
文献类型：
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作者：
Shinjyo N;Nakayama H;Ishimaru K;Hikosaka K;Mi-Ichi F;Norose K;Yoshida H.
金丝桃是东亚传统的重要民族植物药。探讨H.结果表明，该制剂对小鼠肠内寄生虫包囊形成有抑制作用，对小鼠肠内寄生虫包囊形成有抑制作用，对小鼠肠内寄生虫包囊形成有抑制作用。H的成分。用高分辨LC-MS对提取物和溶剂分配分离的组分进行分析，用GFP标记T进行弓形虫生长抑制试验。通过测量荧光强度检测弓形虫PTG-GFP。抗弓形虫药乙胺嘧啶作为阳性对照。通过定量PCR和荧光显微镜，使用PTG-GFP和单核-巨噬细胞系Raw 264的共培养物来评估gondii诱导的免疫反应。以巴龙霉素为阳性对照，用流式细胞仪检测其对侵袭内阿米巴成囊的抑制作用。结果表明，雷公藤甲醇提取物（50 μg/mL）和乙酸乙酯提取物（50 μg/mL）对T.而50%MeOH提取物和亲水性组分则对弓形虫无作用。与T. gondii可降低巨噬细胞的活力，而H则可保护巨噬细胞。甲醇提取物或EtOAc级分（高于10 µg/mL）。甲醇提取物和乙酸乙酯提取物也能有效抑制E. invadensat 1 mg/mL。金丝桃碱是甲醇提取物和乙酸乙酯萃取物中的主要成分，对T. gondigrowth和E.侵入包囊结果表明，H.能有效地抑制弓形虫的生长和内阿米巴包囊的形成。这些活性部分由金丝桃素介导。此外，还表明该提取物和级分可以保护先天免疫细胞免受弓形虫诱导的损伤，从而增强寄生虫清除。进一步的研究是必要的，以解决在体内的有效性。erectumas a anti-protozoal抗原生动物medicine药.
Hypericum erectumis an important ethnobotanical medicine in East Asian tradition. To explore the anti-parasitic potential ofH. erectum, inhibitory effects on the growth of intracellular parasiteToxoplasmaand on the encystation of intestinal parasiteEntamoebawere examined. The constituents inH. erectumalcoholic extracts and fractions separated by solvent-partitioning were analysed by high resolution LC–MS.Toxoplasma gondiigrowth inhibition assay was performed using GFP-labelledT. gondiistrain PTG-GFP by measuring the fluorescence intensity. Anti-Toxoplasmadrug pyrimethamine was used as a positive control.T. gondii-induced immune reaction was assessed by quantitative PCR and fluorescence microscopy, using co-culture of PTG-GFP and monocyte-macrophage cell line Raw264. The inhibitory effect on the encystation ofEntamoeba invadenswas measured by flow-cytometry, where paromomycin was used as a positive control.H. erectummethanol (MeOH) extract (50 µg/mL) and ethyl acetate (EtOAc) fraction (50 µg/mL) inhibited the growth ofT. gondii, while 50%MeOH extract and hydrophilic fractions were ineffective. Co-culture withT. gondiireduced the viability of macrophages, however macrophages were protected in the presence ofH. erectumMeOH extract or EtOAc fraction (above 10 µg/mL). The MeOH extract and EtOAc fraction also effectively suppressed the encystation ofE. invadensat 1 mg/mL. Hypericine, a major constituent in MeOH extract and EtOAc fraction, inhibitedT. gondiigrowth andE. invadensencystation. Our results demonstrated thatH. erectumeffectively inhibitedToxoplasmagrowth andEntamoebaencystation. These activities are partly mediated by hypericin. In addition, it was suggested the extract and fraction may protect innate immune cells fromToxoplasma-induced damages, thereby enhancing parasite clearance. Further investigation is warranted to address the in vivo effectiveness ofH. erectumas an anti-protozoal medicine.