Observations on the behaviour of second stage juveniles of Hetero inside host roots
Observations on the behaviour of second stage juveniles of Hetero inside host roots
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异源性二期幼虫在寄主根内的行为观察
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
U. Zunke
中科院分区：
文献类型：
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作者：
U. Wyss;U. Zunke
摘要利用特殊观察室和高分辨率视频增强对比显微镜，研究了感染沙氏异尖线虫第二期幼虫在甘蓝型油菜幼苗根内的行为。利用这项技术，幼虫在细胞内迁移到合胞体诱导部位，靠近或在血管组织内，并从最初的合胞体取食，可以第一次在活动物身上直接观察到。分析了线虫摄食装置内部结构和最初合胞内结构的功能，这些结构以前只用透射电子显微镜记录。从最初的合胞体中摄取营养总是在一系列重复的循环中进行，每个循环由三个不同的阶段组成：i)通过与口针管腔开口相连的饲养管从修饰的细胞质区域内连续提取营养物质。口针从取食部位回缩，口针在气孔腔内缓慢移动，口针尖端重新插入，伴随着两个食道下腺壶腹部扩张的瓣膜内分泌液的耗尽。(3)从œ食管腺背侧壶腹释放分泌液，在变性细胞质内形成新的饲养管。描述和讨论了在线虫的摄食装置和初始合胞中发生的这三个阶段中发生的事件。
SUMMARY Using special observation chambers and high resolution video-enhanced contrast microscopy, the behaviour of infective second stage juveniles of Heterodera schachtii was studied inside roots of Brassica napus seedlings. With this technique, intracellular migration of the juveniles to the site of syncytium induction near or within the vascular tissue and feeding from the initial syncytium could, for the first time, be directly observed on live animals. The function of structures inside the feeding apparatus of the nematode and in the initial syncytium, previously only recorded with the transmission electron microscope, was analysed. Feeding from the initial syncytium was invariably in a series of repeated cycles, each composed of three distinct phases : i) continuous withdrawal of nutrients from within a zone of modified cytoplasm through a feeding tube connected to the orifice of the stylet lumen. iil stylet retraction from the feeding site, gentle stylet movements within the stomatal cavity and reinsertion of the stylet-tip, accompanicd by depletion of secretory fluids from within the expanded valve membranes of the two subventral oesophageal gland ampullae. iii) release of secretory fluids from the ampulla of the dorsal œsophageal gland, forming a new feeding tube inside the zone of modified cytoplasm. Events that occurred during these three phases within the feeding apparatus of the nematode and in the initial syncytium are described and discussed.