Modular organization of the silkmoth antennal lobe macroglomerular complex revealed by voltage-sensitive dye imaging
Modular organization of the silkmoth antennal lobe macroglomerular complex revealed by voltage-sensitive dye imaging
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DOI：
10.1242/jeb.00784
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发表时间：
2004-02-01
影响因子：
2.8
通讯作者：
Kanzaki, R
中科院分区：
文献类型：
--
作者：
Ai, H;Kanzaki, R
我们成功地阐明了功能性突触组织的巨球复合体（MGC）的雄蚕蛾的光记录与电压敏感染料。触角中的感觉神经元将其轴突沿着内侧神经（MN）或外侧神经（LN）向下发送，这取决于它们是否位于内侧或外侧鞭毛上。在MN中的信息素敏感纤维偏向内侧MGC，而在LN中的偏向外侧MGC在触角叶。在我们的光学记录实验中，在MGC的突触后活动的特点是药理学分析。在MGC中的突触后活动分离的感觉活动在无钙条件下，随后的抑制性突触后活动被分离应用荷包牡丹碱。我们发现，抑制性突触后反应总是在无钙条件下分离的突触后反应之前。此外，兴奋性突触后活动的计算是通过从突触后活动中分离的无钙条件下减去抑制电位。当刺激MN时，中央环、内侧环和内侧丘的兴奋性突触后活动幅度选择性地高于其他区域。相反，当LN被刺激时，兴奋性突触后活动在外侧环和外侧丘的区域被诱发。抑制性突触后活动在整个MGC中均匀分布。这些数据表明，有一个模块化的组织，以MGC，这样的信息从两个主要分支的天线是隔离到不同的子区域的MGC肾小球。
We succeeded in clarifying the functional synaptic organization of the macroglomerular complex (MGC) of the male silkmoth Bombyx mori by optical recording with a voltage-sensitive dye. Sensory neurons in the antennae send their axons down either the medial nerve (MN) or lateral nerve (LN), depending on whether they are located on the medial or lateral flagella. Pheromone-sensitive fibers in the MN are biased towards the medial MGC, and those in the LN are biased towards the lateral MGC in the antennal lobe. In our optical recording experiments, the postsynaptic activities in the MGC were characterized by pharmacological analysis. Postsynaptic activities in the MGC were separated from sensory activities under Ca2+-free conditions, and subsequently the inhibitory postsynaptic activities were separated by applying bicuculline. We found that the inhibitory postsynaptic responses always preceded the postsynaptic responses separated under Ca2+-free conditions. Moreover, the excitatory postsynaptic activities were calculated by subtracting the inhibitory potentials from the posysynaptic activities separated under Ca2+-free conditions. When the MN was stimulated, the amplitudes of the excitatory postsynaptic activities in the central toroid, the medial toroid and the medial cumulus were selectively higher than those in the other areas. By contrast, when the LN was stimulated, excitatory postsynaptic activities were evoked in areas in both the lateral toroid and the lateral cumulus. The inhibitory postsynaptic activities were equally distributed throughout the whole MGC. These data suggest that there is a modular organization to the MGC such that information from the two main branches of the antenna is segregated to different sub-regions of the MGC glomeruli.