Differential regulation of Paramecium ciliary motility by cAMP and cGMP.
Differential regulation of Paramecium ciliary motility by cAMP and cGMP.
复制标题
CAMP和CGMP对纤毛运动的差异调节。
DOI：
10.1083/jcb.106.5.1615
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发表时间：
1988-05
影响因子：
7.8
通讯作者：
Nelson, D L
中科院分区：
文献类型：
--
作者：
Bonini, N M;Nelson, D L
环磷酸腺苷和环磷酸鸟苷对草履虫纤毛运动有明显的调节作用。使用洗涤剂渗透性细胞再活化与MgATP游泳，我们观察环核苷酸和相互作用与Ca 2+对再活化细胞的游泳速度和方向的影响。cAMP和cGMP都使向前游泳速度增加了2 - 3倍，其半最大浓度接近0.5 μ M。然而，这两个环核苷酸，有不同的效果，在拮抗与Ca 2+的反应，向后游泳和手的螺旋游泳路径的再激活的细胞。这些结果表明，cAMP和cGMP差异调节的方向的纤毛动力冲程。
cAMP and cGMP had distinct effects on the regulation of ciliary motility in Paramecium. Using detergent-permeabilized cells reactivated to swim with MgATP, we observed effects of cyclic nucleotides and interactions with Ca2+ on the swimming speed and direction of reactivated cells. Both cAMP and cGMP increased forward swimming speed two- to threefold with similar half-maximal concentrations near 0.5 microM. The two cyclic nucleotides, however, had different effects in antagonism with the Ca2+ response of backward swimming and on the handedness of the helical swimming paths of reactivated cells. These results suggest that cAMP and cGMP differentially regulate the direction of the ciliary power stroke.